Flow Cytometry: First Principles
Alice Longobardi Givan

Wiley-Liss, New York, 1992
10 Chapters, 200 Pages, Glossary, Indexed
ISBN 0-471-56095-2 [Paperback]


Contents

Preface

ix

Acknowledgments

xiii

   

1 The Past as Prologue

1

   

2 Setting the Scene

11

   

3 Instrumentation: Beyond the Black Box

15

The Optical Bench

16

The Fluid System

21

Electronics

28

Sorting

34

   

4 Information Harnessing the Data

41

Data Storage

41

Data Analysis

43

   

5 Seeing the Light: Lasers, Fluorochromes, and Filters

55

General Theory

55

The lIluminating Beam

57

Fluorochromes

60

Lenses, Filters, and Mirrors

66

Compensation

70

   

6 CelIs From Without: Lymphocytes and the Strategy of Gating

75

Lymphocytes

76

Staining for Surface Markers

81

Controls

83

Quantification

88

The Strategy of Gating

90

   

7 Cells From Within: DNA and Molecular Biology

103

Fluorochromes for DNA Analysis

103

Ploidy

104

Cell Cycle Analysis

110

Two-Color Analysis for DNA and Another Parameter

118

Chromosomes

121

Molecular Biology

127

Cell Death

130

   

8 Disease and Diagnosis: The Clinical Laboratory

135

The Hematology/lmmunology Laboratory

138

Oncology/Pathology Laboratories

142

Transplantation

144

Comments

147

   

9 Out of the Mainstream: Research Frontiers

151

Functional Assays

151

The Aquatic Environment

155

Animal Development

159

Microbiology and Gel Microdroplets

161

   

10 Flowing On: The Flow Cytometry Laboratory of the Future

167

   

General References

171

Glossary

175

Figure Credits

193

Index

197