|
Preface |
ix |
|
Acknowledgments |
xiii |
| |
|
|
1 The Past as Prologue |
1 |
| |
|
|
2 Setting the Scene |
11 |
| |
|
|
3 Instrumentation: Beyond the Black Box |
15 |
|
The Optical Bench |
16 |
|
The Fluid System |
21 |
|
Electronics |
28 |
|
Sorting |
34 |
| |
|
|
4 Information Harnessing the Data |
41 |
|
Data Storage |
41 |
|
Data Analysis |
43 |
| |
|
|
5 Seeing the Light: Lasers, Fluorochromes, and Filters |
55 |
|
General Theory |
55 |
|
The lIluminating Beam |
57 |
|
Fluorochromes |
60 |
|
Lenses, Filters, and Mirrors |
66 |
|
Compensation |
70 |
| |
|
|
6 CelIs From Without: Lymphocytes and the Strategy of Gating |
75 |
|
Lymphocytes |
76 |
|
Staining for Surface Markers |
81 |
|
Controls |
83 |
|
Quantification |
88 |
|
The Strategy of Gating |
90 |
| |
|
|
7 Cells From Within: DNA and Molecular Biology |
103 |
|
Fluorochromes for DNA Analysis |
103 |
|
Ploidy |
104 |
|
Cell Cycle Analysis |
110 |
|
Two-Color Analysis for DNA and Another Parameter |
118 |
|
Chromosomes |
121 |
|
Molecular Biology |
127 |
|
Cell Death |
130 |
| |
|
|
8 Disease and Diagnosis: The Clinical Laboratory |
135 |
|
The Hematology/lmmunology Laboratory |
138 |
|
Oncology/Pathology Laboratories |
142 |
|
Transplantation |
144 |
|
Comments |
147 |
| |
|
|
9 Out of the Mainstream: Research Frontiers |
151 |
|
Functional Assays |
151 |
|
The Aquatic Environment |
155 |
|
Animal Development |
159 |
|
Microbiology and Gel Microdroplets |
161 |
| |
|
|
10 Flowing On: The Flow Cytometry Laboratory of the Future |
167 |
| |
|
|
General References |
171 |
|
Glossary |
175 |
|
Figure Credits |
193 |
|
Index |
197 |
