Another kinetic measurement of importance is the change in calcium flux that is apparent in activated cells. The initial steps of signal transduction following receptor-ligand interaction involve activation of phospholipase c and membrane bound glycophotidyl inositol. This leads to the release of inositol phosphates and fatty acids which trigger activation of protein kinase C and subsequent flux of calcium across the plasma membrane. Thus a direct cellular response can be measured if alterations of calcium concentration can be monitored.
