Workshop Tutorial Polish Cytometry Society 1998 Analysis of flow cytometric data - data collection, principles of gating and histogram analysis

10/28/98


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Table of Contents

Workshop Tutorial Polish Cytometry Society 1998 Analysis of flow cytometric data - data collection, principles of gating and histogram analysis

Data Analysis

Data Acquisition

Data Analysis Software

Pros & Cons of Free Flow Software

Flow Cytometer Computer Files

Types of Listmode data

Data Acquisition - Listmode

Listmode File

Listmode data Analysis

One parameter frequency histogram

Establishing Regions

Gating

Real-Time vs. Software Gating

PPT Slide

PPT Slide

PPT Slide

Quadrant Analysis

Quadrant Analysis

Complex or Boolean Gating

Boolean Gating

Boolean Gating

Boolean Gating

PPT Slide

Back Gating

Drawing Regions: Sample Preparation Sample Quality

PPT Slide

Methods that can change results:

PPT Slide

Time as a quality Control Parameter

Conclusion

Author: J. Paul Robinson

Home Page: //flowcyt/educate.htm

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