Basic Theory II


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Table of Contents

Mechanics of a flow cell

How foward angle light scatter is collected

How 90 degree light scatter is collected

Fluorescence detection in the flow cell

Fluorescence activated cell sorting in the flow cytometer

Optical Filters

Mechanism of a band pass filter

Standard Long Pass Filters

Diagramatic representation of the optic layout of a flow cytometer

Common excitation and emission spectra of various fluorescent probes

Excitation and emission spectra of Propidium Iodide

Excitation and emission spectra of Fluorescein (FITC)

Excitation and emission spectra of Phycoerytherin (PE)

Excitation and emission spectra of Allophycocyanin (APC)

Typical Research Cytometer (Coulter 753) (1980s)

Diagram of typical clinical cytometer

The Conventional Microscope

Author: Dr. J. Paul Robinson

Purdue University Cytometry Laboratories

Email: jpr@flowcyt.cyto.purdue.edu

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