PPT Slide

YoYo-1 stained mixture of 70% ethanol fixed

E.coli cells and B.subtilis (BG) spores.

mixture

BG E.coli

BG

E.coli

mixture

Run on Coulter XL cytometer

Scatter

Fluorescence

Scatter

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Yo-Yo 1 - 488 ex membrane impermeant so cells must be fixed.

Fix in ethOH - stain 5 minutes run flow

Spore coat difficult to get dyes in. Spores have less fluorescent than vegetative bacteria