SMALL BEAD
LARGE BEAD
Frequency Histogram
SMALL BEAD
LARGE BEAD
Sample in
Sheath
Sheath in
Laser beam
Stream
Charge
+4KV
-4KV
Waste
SORT RIGHT
SORT LEFT
SORT DECISIONS
Piezoelectric
crystal oscillator
Last attached
droplet
LEFT
RIGHT
Sensors
Sensor
Signals are collected from several sensors placed forward or at 90° to the laser beam. It is possible to “sort” individual particles. The flow cell is resonated at a frequency of approximately 32KHZ by the piezoelectric crystal mounted on the flow cell. T
Figure 1 The central component of a flow cytometer is the flow cell. A cutdown of a typical flow cell indicates the salient features. Sample is introduced via the sample insertion rod. Sheath fluid (usually water or saline) is ntroduced to surround the
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