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4435 N 78th St #277A • Scottsdale, AZ 85251 |
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Phone 480-994-0483 cellphone 310-729-1219 • E-mail biocat2@aol.com |
Mary Price
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Objective |
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Learning new technologies and exploring advancements in science with upward growth potential within an organization with emphasis in running a FACS core.
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Summary of qualifications |
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I am a conscientious, organized, and diligent worker that quickly learns new tasks and concepts. I have extensive experience in FACS technologies, stem cell isolation and culture techniques, retroviral gene transfer with viral vectors, PCR, as well as various other techniques. I enjoy working with people on projects but also can work efficiently on a project independently. I have particular expertise in development and troubleshooting assays. I take pride in my work and can handle multiple projects and tasks with ease.
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Education |
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1986-1990 Bachelor of Science in Biology, Queens College, NC
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Professional experience |
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2004- present St Josephs Hospital Phoenix, AZ FACS Specialist n Operation/Maintenance/Training/Supervision of FACS Aria n CFSE/FACS based assays/Cell culture techniques n Animal work/CFU/Miltenyi cell separations
1996-2004 Childrens Hospital Los Angeles Los Angeles, CA Research Specialist II n Operation and Maintenance of FACS Vantage SE with DiVA option n Operation/Maintenance/Training on BD Calibur and FACScan n Setting up FACS based assays n Stem cell isolation and culture systems n Lentiviral/retroviral supernatant production and transduction of stem cells n Taqman/Southern blotting/PCR assays n CFU assay plating and analysis n PC/MAC--Word/Excel/Powerpoint/Cellquest/DiVA program
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Patents and publications |
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ARTICLES
1. Case S, Price MA, Jordan CT, Yu XJ, Wang L, Bauer G, Haas DL, Xu D, Stripecke R, Naldini L, Kohn DB, and Crooks GM. “Stable Transduction of Quiescent CD34+CD38- Human Hematopoietic Cells by HIV-1 Based Lentiviral Vectors.” Proc Natl Acad Sci USA 96:2988-2993, 1999.
2. Hao Q-L, Zhu J, Price MA, Payne KJ, Barsky LW, Crooks GM. “Identification of a novel, human multi-lymphoid progenitor in cord blood. Blood 97(12): 3683-3690, 2001.
3. George AA, Franklin J, Kerkof K, Shah AJ, Price MA, Tsark E, Bockstoce D, Yao D, Hart N, Carcich S, Parkman R, Crooks GM, Weinberg K. “Detection of leukemic cells in the CD34+CD38- bone marrow progenitor population in children with ALL.” Blood 97(12):3925-3930, 2001.
4. Price MA*, Case SS*, Carbonaro D, Yu XJ, Petersen D, Sabo K, Curran M, Engel B, Margarian H, Abkowitz J, Nolan G, Kohn D, Crooks GM. “Expression from Second Generation Feline Immunodeficiency Virus Vectors is Impaired in Human Hematopoietic Cells.” Mol Ther. 2002 Nov;6(5):645-52.
5. Vollmer TL, Liu R, Price M, Rhodes S, La Cava A, Shi FD. “Differential effects of IL-21 during initiation and progression of autoimmunity against neuroantigen. J Immunol. 2005 Mar 1 ;174(5):2696-701.
6. Zhang Y, Payne KJ, Zhu Y, Price MA, Parrish YK, Zielinska E, Barsky LW, Crooks GM “ SCL expression at critical points in human hematopoietic lineage commitment.” Stem Cells. 2005 Jun-Jul;23(6):852-60.
7. Liu R, La Cava A, Bai XF, Jee Y, Price M, Campagnolo DI, Christadoss P, Vollmer TL, Van Kaer L, Shi FD. “Cooperation of invariant NKT cells and CD4+CD25+ T regulatory cells in the prevention of autoimmune myasthenia.” J Immunol. 2005 Dec 15;175(12):7898-904.
ABSTRACTS
ABSTRACTS1. Price MA,1. Price MA, Thiemann F, Petersen D, Wiltshire L, Crooks GM.“Functional Differences of CD34+ cells defined by Retroviral VectorMarking”. Keystone Symposium, Durango, Colorado, Feb 1997.
2. Price MA, Thiemann F, Carbonaro D, Barsky LW, Pepper K and Crooks GM. “Limitations of Cell Surface Marker Gene Strategies in the Assessment of Retroviral Transduction of Functionally Primitive Hematopoietic Progenitors.” Blood 90(10), Suppl 1:122a, abstract #532, Nov 1997.
3. Hao Q-L, Petersen D, Thiemann FT, Barsky LW, Price MA, Crooks GM. “Primary Human Progenitors With B Lymphoid Potential are Resistant to Retroviral Marking.” Blood 90(10), Suppl 1:263a, abstract #1155, Nov 1997.
4. Case SS, Price MA, Xu D, Bauer G, Naldini L, Verma IM, Kohn DB, and Crooks GM. “Stable Transduction of CD34+/CD38- Cells in Extended Long Term Culture Using a Lentiviral Vector Pseudotyped with the Vesicular Stomatitis Virus Envelope Glycoprotein G.” Keystone Symposium, Incline Village, Nevada, Jan 1998.
5. Price MA, Thiemann FT, Carbonaro D, Barsky LW, Pepper K, Kohn DB and Crooks GM. “Functional and Molecular Analysis of TransducedHematopoietic Progenitors Defined on the Basis of Vector Expression.” American Society of Gene Therapy ASGT, Seattle, Washington, abstract #353, 1998.
6. Case SS, Price MA, Xu D, Bauer G, Naldini L, Verma IM, Kohn DB and Crooks GM. “Stable Transduction of CD34+/CD38- Cells in Extended Long Term Culture Using a Lentiviral Vector.” American Society of Gene Therapy (ASGT), Seattle, WA, abstract #413, 1998.
7. Crooks GM, Case SS, Price MA, Naldini L and Kohn DB. “Efficient Transduction of CD34+CD38- with Lentiviral Vectors.” Exp Hematol 26(8):815, abstract #478, 1998.
8. Crooks GM, Price MA, Jordan CT, Bauer G, Xu D, Yu XJ, Verma IM, Naldini L, Kohn DB. “Transduction of CD34+CD38- Cells With Lentiviral Vectors.” Blood 92(10) Suppl 1: abstract #2140, 1998.
9. Case SS, Price MA, Xu D, Bauer G, Stripecke R, Verma IM, Naldini L, Kohn DB and Crooks GM. “Efficient Transduction and Stable Gene Expression of CD34+/CD38- Cells With Lentiviral Vectors.” Fourth Cold Spring Harbor Meeting on Gene Therapy in Cold Spring Harbor, NY, September 1998.
10. Kohn DB, Case S, Haas D, Price MA, Xu D, Arevalo J, Rice CR, Halene S, Naldini L, Verma I, and Crooks GM. “Lentiviral Vectors Show Improved Gene Transfer in Human Hematopoietic Stem Cells.” Keystone Symposium, Salt Lake City, UT, 1999.
11. Price MA, Zhu JY, Barsky LW, Jang T, Crooks GM. “GALV and Amphotropic Receptor Expression in Human Hematopoietic Subset.” American Society of Gene Therapy (ASGT), Washington, DC, Abstract #267, 1999.
12. George A, Franklin J, Kerkof K, Price MA, Tsark E, Yao D, Carcich S, Parkman R, Crooks GM, Weinberg K. “Variable contamination of the CD34+CD38- Progenitor Population by Leukemic Cells in Children with ALL.” Blood 94(10), Suppl 1: Abstract #631, 1999
13. Hao Q-L, Zhu J, Price MA, Payne KJ, Wang X, Barsky LW, Nolta JA, Crooks GM. “CD7 expression defines a CD34+CD38- subpopulation with differentiation potential restricted to lymphoid lineages.” Blood 96(11):662a, abstract #2849, 2000.
14. Hao Q-L, Zhu J, Price MA, Payne KJ, Wang X, Yao D, Barsky LW, Nolta JA, Weinberg KI, Crooks GM. “Differential expression of the common gamma chain (gc) on CD34+CD38- cells allow identification of a quiescent, pluripotent subpopulation.” Blood 96(11):667a, abstract #2870, 2000.
15. Case SS, Price MA, Sabo KM, Margarian H, Curran MA, Engel BC, Yu X-J, Zhang Y, Haas DL, Nolan GP, Abkowitz JL, Kohn DB, Crooks GM. “Transduction of human hematopoietic stem cells is more efficient by HIV-1-based lentiviral vectors than by vectors based on FIV.” American Society of Gene Therapy 2001 meeting, Seattle, Washington.
16. Payne KJ, Dovat S, Holly-Nicolas JL, Zhu JY, Bockstoce DC, Barsky LW, Price MA, Smale ST, Crooks GM. “Multiple Ikaros splice variants previously linked to leukemia are expressed in normal human cord blood and bone marrow.” Keystone Symposia on Hematopoiesis, Whistler, British Columbia, Canada, 2001.
17. Hao Q-L, Zhu, J, Price MA, Barsky LW, Zhang J, Gruber J, Payne KJ, Kohn DB, Crooks GM. “Tracking the origins of human dendritic cells using lineage specific progenitors.” Submitted to annual meeting of the American Society of Hematology, 2001.
18. Wang X, Ge S, Crooks GM, Hao Q-L, Price MA, Nolta JA. “Development of human albumin-expressing cells in the liver of immune deficient mice transplanted with highly purified human hematopoietic stem cells.” Submitted to annual meeting of the American Society of Hematology, 2001.
19. Hao QL, Zhu J*, Price MA*, Payne KJ, Zhang J*, Barsky L, Crooks GM. “Differential Expression of CD7 Identifies B/NK and B lymphoid Progenitors in Human Bone Marrow.” Submitted to the annual meeting of the American Society of Hematology, 2002.
20. Timothy L. Vollmer*, Ruolan Liu*, Mary Price*, Susan Rhodes*, Antonio La Cava†, Fu-Dong Shi*2 “Contribution of IL-21 in autoimmunity against neuroantigen.” 19th CMSC Conference 2005
21. Ruolan Liu1, Antonio La Cava2, Mary Price1, Susan N. Rhodes1, Denise I. Campagnolo1, Timothy L. Vollmer1, Nora Sarvetnick3, Fu-Dong Shi1 “Bilateral regulation of NK cells and dendritic cells necessary for genesis of autoimmunity.” 19th CMSC Conference 2005
22. Timothy L Vollmer, Ruolan Liu, Mary Price, Susan Rhodes, Antonio La Cava, Dr. Fu-Dong Shi. “Differential effects of IL-21 during initiation and progression of autoimmunity against neuroantigen.” 57th Annual Meeting of the American Academy of Neurology, 2005
23. Ruolan Liu, Antonio LaCava, Pollard K Michael, Mary Price, Susan N Rhodes, Timothy L Vollmer, Nora Sarvetnick, Fu-Dong Shi. “Impaired Neuroantigen Priming Determines Resistance to the Induction of Autoimmunity in MyD88-Deficient mice”. 57th Annual Meeting of the American Academy of Neurology, 2005
24. Ruolan Liu, Antonio La Cava, Xue-Feng Bai, Youngheun Jee, Denise Campagnolo, Mary Price , Premkumar Christadoss, Timothy Vollmer, Luc Van Kaer, Fu-Dong Shi. “Cooperation of iNKT Cells and CD4+CD25+ Treg Cells in the Prevention of Autoimmune Myasthenia”. Keystone Symposium 2006
25. Timothy L Vollmer*, Ruolan Liu*, Young-Huen Jee*, Wenhua Piao*, Mary Price*, Susan Rhodes*, Denise I Campagnolo*, Mary Collins†, Deborah A Young†, Luc Van Kaer‡, & Fu-Dong Shi*. “NK cell degeneration in multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE).” CMSC 2006
26. Youngheun Jee, Ruolan Liu, Mary Price, Denise I. Campagnolo, Fu-Dong Shi, Timothy L. Vollmer. “CD4+CD25+ regulatory T cells contribute to the therapeutic effects of glatiramer acetate in experimental autoimmune encephalomyelitis”. FOCIS and CMSC 2006
27. Ruolan Liu, Antonio La Cava, Xue-Feng BaiYoungheun Jee, Mary Price, Denise Campagnolo, Premkumar Christadoss, Timothy Vollmer, Luc Van Kaer, Fu-Dong Shi. “Cooperation of iNKT Cells and CD4+CD25+ Treg Cells in the Prevention of Autoimmune Myasthenia”. 58th Annual Meeting of the American Academy of Neurology 2006
28. Lora W. Barsky, Ewa Zielinska, Mary A. Price, Kimberly J. Payne, Yanjia Zhang, Qian-Lin Hao, Yuhua Zhu, Yasmin K. Parrish, Kenneth I. Weinberg and Gay M. Crooks. “Practical Strategies for Succesful Rare Event Detection and Isolation of Human Hematopoietic Stem Cell Populations with Flow Cytometric Instrumentation.” ISAC 2005
29. Amit Bar-Or, Jack Antel, Caroline A Bodner, Denise Campagnolo, Jill Gianettoni, Farzaneh Jalili, Norman Kachuck, Yves Lapierre, Masaaki Niino, Joel Oger, Mary Price, Susan Rhodes, Fu-Dong Shi, Lawrence Steinman, Frank Valone, Timothy Vollmer, Leslie Weiner, Hideki Garren. “Antigen-specific immunomodulation in multiple sclerosis patients treated with MBP encoding.” 58th Annual Meeting of the American Academy of Neurology 2006
30. H. Garren, J. Antel, A. Bar-Or, C. Bodner, D. Campagnolo, J. Gianettoni, F. Jalili, N. Kachuck, Y. Lapierre, M. Niino, J. Oger, M. Price, S. Rhodes, F. Shi, F. Valone, T. Vollmer, L. Weiner, L. Steinman. “Phase I/II trial of a MBP encoding DNA plasmid (BHT-3009) alone or combined with atorvastatin for treatment of multiple sclerosis.” 16th Meeting of the European Neurological Society May 2006
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References |
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Gay M Crooks, MD Associate Professor, Keck school of Medicine University of Southern California, Department of Pediatrics Childrens Hospital Los Angeles (323) 669-5690
Kimberly Payne, PhD Assistant Professor of Research USC Keck School of Medicine Childrens Hospital Los Angeles Division of Research Immunology & Bone Marrow Transplantation (323) 669-5620
Lora Barsky Flow Cytometrist, Supervisor Core Facility Childrens Hospital Los Angeles (323) 669-5935
Gerhard Bauer Laboratory Director, GMP Facility Washington University School of Medicine, Division of Oncology St. Louis, MO 63110 (314) 362 9011
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