October 18, 2007
CURRICULUM VITAE
Name: Gary A. Stone
Date of Birth: December 1, 1952
Home Address: 2906 Jefferson Pike
Jefferson, MD 21755
(301) 834-3020
Work Address: NCI-FCRF, CCR, MCGP, CMCC
Building 560, Room 11-43
P.O. BOX B
Frederick, MD 21702-1201
Phone: (301) 846-5229 E-mail: gstone@ncifcrf.gov
Education:
1975-1978 Shepherd College, Shepherdstown, WV
Major- Biology
1978-1979 B.S. (Biology) Marshall University, Huntington, WV,
1980-1982 MBA Candidate Marshall University, Spring 1980,
core courses completed as follows: Business
Law, Commercial Law, Principles of Marketing,
Principles of Management, Principles of Accounting
I and II.
1982-1986 University of Illinois, Champaign-Urbana, IL-
Electron Microscopy Course, June, 1984.
NIH Radiation Class, January, 1985
Advanced Flow Class, Ortho, Westwood, MA, July, 1985.
Flow Cytometry Workshop, DuPont, Wilmington, DE
1990-Present HIV Training Class, December, 1990.
Hazardous Materials Transportation Training Class June,
Appointed NINDS Safety Committee November, 1997
Chromosome Analysis and Sorting Clinic February, 2000
Los Alamos National Laboratory
Experience:
1999 to Present Biologist, Core Manager, NCI-FCRF, CCR, CMCC
As a Senior Research Associate with the Comparative Molecular Cytogenetics Core, I am responsible for managing the Core, Chromosomal preps, sorting chromosomes, primary and secondary PCRs of sorted probes, FISH, property officer and lab safety representative. As core manager I am responsible for the functioning of the lab. From request submissions to work completions, I follow the flow of the specimens to ensure a timely turn around for submitted samples. I also make sure the lab equipment is maintained and the lab is stocked with supplies. Chromosome sorting is one of my more important functions in the core. I set-up, align and verify the sorter system every morning for the daily sort procedures. I write and initiate the computer protocols for various programs for specific investigator’s requests. I screen sample preps as a prerequisite to chromosome sorts both bulk and initial. These then go to probes to analyze translocations and abnormalities of the flow karyotype. I do the primary PCR for the entire core utilizing DOP PCR technique which yield probes and secondary products. I also grow and extract plasmids from BAC libraries for future probes. I initiate and negotiate the MTA’s for required samples for probes that leave the NCI. I advise investigators about the abilities and tools the Core has and how they might be applied to their research. I look for novel approaches and applications for cancer research.
11/1999 Transferred from NINDS to NCI
1988-11/1999 Biologist, LCNSS, DIR, NINDS, NIH. In the flow cytometry lab, I was the sole operator of the Ortho System 50-H Cytoflurograf cell sorter and the Becton Dickinson three color FACScan instruments. In this capacity, I would set up, stain and analyze specimens for immunophenotyping. I set up immunopanels for primates. I also set-up and ran protocols for cell sorting specimen populations by cell cycle analysis or monoclonal antibodies. I also performed virus isolation and identification by co-cultivation of specimens with known cell lines, P-24 or P-19 antigen cap-ture assay to detect active viruses in culture. Western blotting of serum
and plasma for virus identification. Particle gel agglutination assays were used when cross speciation of samples was a problem for virus identification. I was responsible for policies and procedures of BL-3 and lesser rated laboratories at this facility. I was responsible for the disposition, inventory and disposal of accumulated biological specimens and equipment at the closing of the building. Also at LCNSS, I performed restriction fragment length polymorphism endonuclease assays. I also performed TSE (transmissible spongiform encephalopathies) determinations. I used estriction endonucleases for detection of mutations on codons 100, 102, 129, 178, 187, 200 and 210 located on the PrP region.
1985-1988 Biologist, Infectious Diseases Branch,
IRP, NINCDS, NIH
April 1985, GS-9, October 1987, GS-11
Lower primate panels, AZT protocols, HIV screens, HIV and HTLV immunopanels System 50 Cytofluorograf dual laser.
1980-1985 Marshall University School of Medicine, Huntington, WV
Electron microscopist, autopsies, TEM techniques, atherosclerotic research, System 50 cytofluorograf.
Military Service:
1971-1975 After four years active duty in the U.S. Air Force, with tours in Texas, the
Philippines and Arkansas, I was honorably discharged in June of 1975.
Military awards and medals include the National Defense Service Medal,
Presidential Unit Citation with Detail Unit Citation, Philippine Presidential
Unit Citation and Air Force Good Conduct Award.
I am a 10 point service connected disabled veteran.
Awards:
Quality Step Increases, Special Act awards, Promotions
BIBLIOGRAPHY
1. Stone,G.A., Johnson, B.K., Druihlet, R., Garza, P.B. , and Gibbs, C.J. Jr. 2000) Immunophenotyping of peripheral blood, ranges of serum chemistries and clinical hematology values of healthy chimpanzees (Pan troglodytes) J Med Primatol 2000: 29: 324-329.
2. Cavagna, P., Stone, G., and Stanyon, R. Black rat (rattus rattus) genomic variability characterized by chromosome painting. Mamm Genome 2002 Mar; 13(3): 157-63.
3. Stanyon, R, Stone, G., and Bigoni, F. The ancestral genomes in primate phylogeny and origins: a molecular cytogenetic perspective. (Eds Ross C, Kay R.) Kluwer Academic/Plenum Publishers NY.
4. Stanyon, R., Stone, G., Garcia, M., and Froenicke, L. Reciprocal chromosome painting shows that squirrels, unlike murid rodents, have a highly conserved genome organization. Genomics. 2003 Aug; 82(2):245-9.
5. Fronicke, L., Wienberg. J., Stone, G., Adams, L., and Stanyon, R. Towards the delineation of the ancestral eutherian genome organization: comparative genome maps of human and the African elephant (Loxodonta africana) generated by chromosome painting. Proc Biol Sci. 2003 Jul 7; 270 (1522): 1331-40. Erratum in: Proc R Soc. Lond B Biol Sci. 2003 Dec 22; 270 (1533): 2639.
6. Svartman, M., Stone, G., Page, J.E., and Stanyon, R. A chromosome painting test of the basal eutherian karyotype. Chromosome Res. 2004; 12 (1):45-53.
7. Stanyon, R., Bigoni, F., Slaby, T., Muller, S., Stone, G., Bonvicino, C.R., Neusser, M., and Seuanez, H.N. Multi-directional chromosome painting maps homologies between species belonging to three genera of New World monkeys and humans. Chromosoma. 2004 Dec; 113 (6): 305-15.
8. Bigoni, F., Stone, G., Perelman, P., and Stanyon, R. Cytotaxinomy of Colobinae Primates with Reference to Reciprical Chromosome Painting of Colobus guereza and Humans. Fierenze University Press, August 29, 2004, pp 19-32.
9. Stanyon, R., Bruening, R., Stone, G., Shearin, A., and Bigoni, F. Reciprocal painting between humans, De Brazza's and patas monkeys reveals a major bifurcation in the Cercopithecini phylogenetic tree. Cytogenet Genome Res. 2005; 108(1-3): 175-82.
10. Dumas, F., Bigoni, F., Stone, G., Sineo, L., and Stanyon, R. Mapping genomic rearrangements in titi monkeys by chromosome flow sorting and multidirectional in-situ hybridization. Chromosome Res. 2005; 13(1): 85-96.
11. Svartman, M., Stone, G., and Stanyon, R. Molecular cytogenetics discards polyploidy in mammals. Genomics. 2005 Apr; 85(4): 425-30.
12. Stanyon, R., Bruening, R., Stone, G., Shearin, A., and Bigoni, F. Reciprocal painting between humans, De Brazza's and patas monkeys reveals a major bifurcation in the Cercopithecini phylogenetic tree. Cytogenet Genome Res. 2005; 108(1-3): 175-82.
13. Stanyon, R., Dumas, F., Stone, G., and Bigoni, F. Multidirectional Chromosome Painting Reveals a Remarkable Syntenic Homology Between the Greater Galagos and the Slow Loris. Am J Prim 2005: 67: 1-11.
14. Buckley-Beason, V., Johnson, W., Nash, W. Stanyon, R. Menninger, J. Driscoll, C., Howard, J., Bush, M., Page, J., Roelke, M., Stone, G., Martelli, P., Wen, C., Ling, L., Diraisingham, R., Lam, P., and O'Brien, S.J. Molecular Evidence for Species-Level Distinctions in Clouded Leopards, Current Biology: 16, 2371-2376, December 5, 2006.
15. Kellogg, M., Burkett, S., Dennis, T., Stone, G. Gray, B., McGuire, P., Zori, R., and Roscoe Stanyon. Chromosome Painting in the Manatee Strongly Supports Afrotheria and Paenungulata. BMC Evolutionary Biology 2007, 7: 6, 23. January 2007.
16. Francesca Dumas, Roscoe Stanyon, Luca Sineo, Stone, G., and Bigoni, F. Phylogenomics of species from four genera of New World monkeys by flow sorting and recipricol painting. BMC evolutionary Biology 2007, 7(Suppl 2): S11.
Published abstracts, Laboratory Demonstrations and Proceedings: Since 2000
2001 NCI-Frederick?Ft. Detrick Spring Research Festival
Cetacean Creation by Fronicke, Adams, Stone, Wienberg and Stanyon
Comparative molecular Cytogenetics Coreat NCI-CCR by Adams, Bigoni, Mazzei, Stanyon and Stone
Comparative Genomics in Horse and other Perissodactyls Revealed by Recipricol Chromosome Painting by Shearin, Stone, Fronicke and Stanyon
2007 NCI-Frederick/Ft. Detrick Spring Research Festival
Comparative Molecular Cytogenetics Core at NCI-Frederick, CCR by Burkrtt, Stone and Tessarollo. 20th NIH Research Festival Comparative Molecular Cytogenetics Core at NCI-Frederick, CCR by Burkrtt, Stone and Tessarollo.
References will be provided upon request.