RE: Calcium analysis

Eric Martz (emartz@microbio.umass.edu)
Mon, 12 Feb 1996 11:13:12 -0500 (EST)

In response to Eric Miller's query about calcium on a FACScan:

Either of the free packages WinMDI (Windows) or MFI (PC) will plot
a parameter vs. time. MFI has some additional kinetics capabilities,
including calculating a ratio, and plotting the median value vs. time
or vs. event number (if your acquisition software doesn't acquire time
as a parameter). You'd need to move your listmode data from the HP
to a PC. My catalog of free flow cytometry software has info on the
options available for doing this:
http://www.bio.umass.edu/mcbfacs/flowcat.html

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Eric Martz, Professor of Immunology emartz@microbio.umass.edu
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