There will be a spectral shift depending on the distance of the
fluorophores. E.g. FITC-Emission generated by an PLA1
alloantibody will be absorbed by an anti-GpIIb/IIIa monoclonal
depending on distance of the epitopes. The method can
be used for the characterization of human anti-platelet
antibodies. We published the method in
J. Immunol. Methods 187: 53-67, 1995.
Gregor Rothe
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Institute for Clinical Chemistry and Laboratory Medicine
University of Regensburg
D-93042 Regensburg, F.R. Germany
Tel. +49 (941) 944-6204
Fax +49 (941) 944-6202
Internet: Gregor.Rothe@klinik.uni-regensburg.de
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