>
> I am hoping that someone can give me some (any!) information on the effect of
> chymopapain on surface markers.
Some are clipped, some are not. I would recommend incubating cell lines
and testing to see how things are affected in your own system.
>
> Another related question, does anyone know a gentle method of removing
> antibody from CD34?
Aside from chymo, cedarlane markets a sialoprotease which works well.
Dynal has a nice system with mag beads and "detachabead" anti-FAB frags
which works great if you can afford it.
Steve Gore
Johns Hopkins Oncology Center