Re:PI and 3 colour mouse lymphs

Mikael Vestberg (Mikael.Vestberg@inflam.lu.se)
Wed, 28 Feb 96 17:20:38 +0100

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We have used anti CD8a-Red613 or anti-B220-Tricolour with PI. By
titrating the amount of PI used, you can separate the population from
the antibody stained ones. Usually PI staining is 5-10x brighter. In the
first case the PI+ will show up in the (red613-)corner of a PE/red613
plot, but will appear as a double positive population if you use TC as a
third colour, since less compensation is needed. We usually stain lymphs
and set amplifiers to find them around 7x10e2 on our FACSort-Cellquest
set up. PI positives are usually found above 3x10e3.

Mikael

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