Try Mitomycin C to arrest your cells. It should do the trick. Need a protocol? email me direct.
>Hi
>
>All you cell cycle experts out there, can any one suggest ways of blocking
>cell cycle without killing cells. We would like our cells (T-cell lines) to
>remain arrested for several days without dying. We need the cells to be in
>G1, S or G2. We have tried gamma radiation and aphidicoline but the cells
>do not survive to the end of the assay.
>
>Simon Monard
>Aaron Diamond Center
>New York
***********************************************
Roger A. Burger E-mail: Roger@cpd2.usu.edu
Research Immunologist
Center for Persons with Disabilities
Utah State University
Logan, UT 84322-6895
Voice: 801-797-2042
FAX: 801-797-2044