Does anyone have any experience in assaying intracellular
benzyloxycarbonyl tetra peptide AFC (7-Amino-4-Trifluoromethyl
Coumarin substrates.A few simple questions follow.
1 Are these compounds generally cell permeable
2 Can the cleaved AFC be excited at 352 nm , if so what is
the emission wavelength.
3 Does the cleaved AFC diffuse out of the cell.
Thanks for any help
Roger Snowden
rts1@le.ac.uk