JCSMR Flow Cytometry (FACS Lab.)
Texts Page
Last updated : 11 Sept. 95
The creator of this page:Geoff.Osborne@anu.edu.au
Index of Texts available in the lab.
- Methods Texts
- Reference Texts
- Some of the Catalogues (commercial) available for loan
- Flow Cytometry Course manuals
"Handbook of Flow Cytometry Methods."
J.Paul Robinson, Editor
- Associate Editors:
- Zbigniew Darzynkiewicz,
Phillip Dean,Lynn Dressler,
Hans Tanke, and Leon Wheeless.
Major Contents headings of this text are:
Standards, Reagents, & Instruments
Precautions
Methods
- Phenotypic Analysis
- Measurement of DNA
- Other General Flow Methods
- Methods for Functional Assessment
- Associated Methods
Data Analysis & Computers
Lab Comments:Handbook of Flow Cytometry
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"Flow Cytometry: A Practical Approach."
M.G.Ormerod, Editor
Major Contents headings of this text are:
1. Introduction to the principles of flow cytometry
2. An introduction to fluorescence technology
3. Measurement of cellular subsets using antibodies
4. Analysis of DNA
- 4A. General Methods
- 4B. Measurement of tumour cell kinetics by Bromodeoxyuridine method.
- 4C. Cell kinetic analysis using continuous BrdU labelling and bivariate Hoechst 33358/Ethidium Bromide flow cytometry.
5. Cell Sorting
- 5A. Using the Coulter Epics C
- 5B. Using the Becton Dickinson FACS for sorting
- 5C. On the Ortho Cytofluorograf
6. Chromosome analysis and sorting
7. Intracellular ionized calcium membrane
8. Flow cytometry of RNA content
9. Nuclear associated antigens
10. Analysis of radiation and chemical damage in mammalian cells
11. Measurement of dynamic cellular events
12. Further applications to cell biology
Lab Comments:Flow Cytometry: A Practical Approach
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List of some of the Commercial catalogues available in the lab.
These catalogues are available for loan, with some providing extensive information and references. THESE CATALOGUES ARE IN NO WAY RECOMMENDATIONS OF A PARTICULAR COMPANY OR A PARTICULAR COMPANIES PRODUCTS.
Becton Dickinson Reagent Catalog
Bioclone Product Catalogue
Coulter Cytometry Catalog
Caltag Laboratories: Catalog 1995-1996
Dako 1995 Catalog
Endogen Inc. Product Reference Guide
Pharmingen Research Products Catalog 1994-95
R&D Systems 1995 Catalog
Serotec 1995/96 Product Guide
Zymed Laboratories Inc. Immunochemical Products 1994-1995 Catalog
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Flow Cytometry Course Manuals
There are a number of course manuals available in the laboratory. These manuals contain a wealth of methods, in different aspects of flow.
The latest addition to these manuals is the manual from the National Course in Flow Cytometry, Los Alamos, New Mexico, USA. This looseleaf book is some 12 cm. thick and even covers such areas as "How to build a flow cytometer". For more detailed information on the contents of these manuals, drop pass, drop us an email, or phone.
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