Deutsche Gesellschaft für Zytometrie
(DGZ)

DGZ

The abstract are printed in the: Proceedings of the DGZ Heidelberg Meetings, DKFZ, Heidelberg 1995, ISSN 0949-5347

=== ORAL PRESENTATIONS ===

1. CELL KINETIC EFFECTS INDUCED BY INTERFERON GAMMA AND TUMOR NECROSIS FACTOR ALPHA IN HUMAN TUMOR CELLS, INVOLVEMENT OF CYCLINS
H. Baisch
Institute of Biophysics and Radiobiology, University of Hamburg, Martinistrasse 52, D-20246 Hamburg

2. ROLE OF PROTEIN KINASE ACTIVITY IN REGULATION OF CELL CYCLE PROGRESSION
H. Crissmann, J. Valdez, S. Rose, S. Minter
Life Sciences Division Los Alamos National Laboratory, USA

3. STROMA ORIGINATING FROM SORTED CD13-POSITIVE BONE MARROW CELLS
J.W. Ellwart, K. Nispel, R.A.J. Oostendorp, G. Ledderose*, P. Dörmer
GSF-lnstitut für Experimentelle Hämatologie, München, *Med. Klinik III, Klinikum Grosshadern, Universität München

4. CELL CYCLE KINETICS OF UROTHELIAL CELL LINES AS MEASURED BY FLOW CYTOMETRY USING BRDU/HOECHST TECHNIQUES AND IMMUNHISTOCHEMICAL STAINING FOR PROLIFERATION ASSOCIATED ANTIGENS
E. Endl, G. Fauser, P. Steinbach, R. Knüchel and F. Hofstädter Institute of Pathology, Franz-Josef-StrauB Allee 11, D-93053 Regensburg

5. DETECTION OF MINIMAL RESIDUAL DISEASE IN ACUTE MYELOGENOUS LEUKEMIA AND MYELODYSPLASTIC SYNDROMES IN CLINICAL REMISSION BY FACS/FISH
H. Engel,1 A. Gooodacre, A. Keyhani, S. Jiang and M. Andreeff
Department of Hematology, University of Texas M. D. Anderson Cancer Center, Houston, USA and1 Institiut für klinische Biochemie der Universität Bonn, Germany

6. ANALYSIS OF PARTICLE-SIZE FOR TESTING ON CYTOTOXICITY
D. Fieblinger, K. Grünheid, D. Labes
Pharmakologische Forschungsgesellschaft BIOPHARM GmbH Berlin, Alfred-Kowallke-Strasse 4,10315 Berlin

7. ANGULAR RESOLVED LIGHT SCATTERING OF SINGLE PARTICLES AND PARTICLE AGGLOMERATES IN A FLOW CYTOMETER
C.Gohlke, J.Neukammer, and H.Rinneberg
Physikalisch-Technische Bundesanstalt, Berlin-Charlottenburg, Abbestrasse 2-12, D-10587 Berlin,Germany

8. FLOW CYTOMETRIC S-PHASE DETERMINATION IS A STRONGER PROGNOSTIC FACTOR IN NODE-NEGATIVE BREAST CANCER THAN IMMMUNOCYTOCHEMICAL MIB1 (Ki-67) STAINING
N. Harbeck, P. Dettmar1, C. Thomssen, L. Pache, M. Schmitt, F. Jänicke, W. Nathrath1, and H. Graeff
Frauenklinik and 1 Institut für Pathologie, Technische Universität, D-81675 München, Germany.

9. MODEL SYSTEM FOR ISOLATION OF COMPETENT OVARIAN CARCINOMA CELLS FROM FRESH TUMOR TISSUE BY A MAGNETIC SEPARATION SYSTEM (MACS)
N. Harbeck, A. Abdulsalam, S. Schwarze, E. Schüren, P. Dettmar1, W.Kuhn, H. Graeff, and M. Schmitt
Frauenklinik and 1 Institut für Pathologie, Technische Universität, M&u uml;nchen, Germany.

10. INFLUENCE OF FLUORESCENT LABELING OF GLYCOSAMINOGLYCANS (GAGs) FOR BINDING TO LEUKOCYTES
J. Harenberg. R. Malsch. L. Piazolo. G. Huhle. and D.L. Heene
1st Department of Medicine, Faculty for Clinical Medicine Mannheim of the University Heidelberg, Theodor-Kutzer-Ufer, D-68167 Mannheim

11. SERIAL FLOW CYTOMETRY DURING CHEMOTHERAPY: IMPACTS ON THE DEVELOPMENT OF INDIVIDUALIZED TREATMENT STRATEGIES
J. Hemmer
University of Ulm, Germany, Division of Tumor Biology

12. DIFFERENT APPROACHES TO STUDY THE NUCLEOLAR TERRITORY DURING THE CELL CYCLE
D. Hernandez-Verdun, C. Masson and R. Junera
Institut Jacques Monod, 2 place Jussieu, 75251 Paris Cedex 05, France

13. QUANTITATIVE ANALYSIS AND QUALITY ASSURANCE FOR FLOW CYTOMETRY
H.-G. Höffkes und G. Schmidtke
Abteilung für Hämatologie, Zentrum fur Innere Medizin, Universität Essen, Hufelandstrasse 55, 45122 Essen

14. ISOLATION AND CHARACTERIZATION OF PERIPHERAL BLOOD DENDRITIC CELLS BY HIGH-GRADIENT MAGNETIC CELL SORTING
Alexander Horst*, Andreas Radbruch#, Stefan Miltenyi* and Jürgen Schmitz*
* Miltenyi Biotec GmbH, Bergisch-Gladbach, Germany and #Institute for Genetics, University of Cologne, Cologne, Germany

15. COMBINATION OF B-CELL PURGING AND CD34+ SELECTION FOR LYMPHOMA CELL REMOVAL FROM BLOOD STEM CELL AUTOGRAFTS
P. Hundsdörfer1, S. Frühauf2, R. Höft1, R. Haas2, W. J. Zeller1
1 German Cancer Research Center, Research Program Diagnostics and Experimental Therapy, Heidelberg, Germany, 2 Department of Internal Medicine V, University of Heidelberg

16. A NEW 3-COLOUR LYSE-NO-WASH (LNW), ABSOLUTE COUNT SYSTEM FOR IMMUNOPHENOTYPING AND HIV MONITORING
Luc Ketele
Becton Dickinson Immuncytometry Systems Europe, Erembodegem, Belgium

17. "TERBIUM-NUCLEOTIDES" FOR LUMINESCENT LABELLING AND SENSITIVE TIME-RESOLVED DETECTION OF NUCLEIC ACIDS
S. Klingel, M. Collasius and G. Valet
Arbeitsgnuppe Zellbiochemie, Max-Planck-lnstitut für Biochemie, D-82152 Martinsried

18. IMPACTS OF THE DNA PLOIDY STATUS OF THE PRIMARY TUMOR OKKULT METASTASIS IN ORAL SQUAMOUS CELL CARCINOMA
K. Kraft*, A Schouba#, J.Hemmer#
*Dept of Pathology, Military Hospital Ulm, Germany,# Division of Tumor Biology. University of Ulm, Germany

19. DIFFERENTTIATION OF OVARIAN TUMORS WITH IMAGE ANALYSIS
W. Kuhn, M. Ruhnke, A. Ditzenbach, H. Weitzel
Department Gynecol. Obstet., Klinikum Benjamin Franklin Free University of Berlin, Germany

20. NEW DEVELOPMENTS IN CONFOCAL LASER SCANNING MICROSCOPY
J.Kukulies
Carl Zeiss Jena GmbH, Unternehmensbereich Mikroskopie, Systemvertrieb Deutschland

21. DETECTION OF MDR-1 mRNA BY RT-PCR AND FLOW CYTOMETRY
D. Lassner, J. Milde, M. Ladusch*, K. Drössler* and H. Remke
Institute of Clinical Chemistry and Pathobiochemistry, *Institute of Zoology, University Leipzig, Liebigstrasse 16, D-04103 Leipzig

22. COMPARISON OF BrdU INCORPORATION AND PCNA AND Ki67 EXPRESSION IN HL-60 CELLS AFTER INDUCTION AND IRRADIATION
M. Lindinger*, A. Sendler**, K.-P. Gilbertz*, D. van Beuningen*
*Institut für Radiobiologie, Akademie des Sanitäts- und Gesundheitswesen der Bundeswehr, D-80901 München, **Chirurgische Klinik und Poliklinik der TU München, Klinikum "rechts der Isar", D-81644 München, Germany.

23. COMPARISON OF FLOW CYTOMETRIC METHODS FOR THE ANALYSIS OF APOPTOTIC CELLS
Kristin Marx, Michael Nüsse
GSF-Forschungszentrum für Umwelt und Gcsundheit, AG Durchflußzytometrie, D-85758 Oberschleißheim, Germany

24. FLOW SORTING OF PRINS LABELLED PLANT CHROMOSOMES
Armin Meister, Uta Pich and Ingo Schubert
Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben

25. PROGNOSTIC RELEVANCE OF PLOIDY AND S-PHASE IN RESECTED CARCINOMA OF THE PANCREAS
H. Nekarda, J.D. Roder, K. Becker*, A. Rossmann, J.R. Siewert
Department of Surgery and *Institut for Pathology, Technische Universität München, Klinikum rechts der Isar

26. DEMONSTRATION OF THE NUMBER OF RESIDUAL LEUCOCYTES OR PLATELETS IN FILTRATED RED BLOOD CELL CONCENTRATES AND PLASMA PREPARATIONS
J. Neumüller, *D.W.M. Schwartz, *W.R. Mayr
Ludwig Boltzmann Institute for Rheumatology and Balneology, Vienna, Austria *Blood Donation Center, Austrian Red Cross, Vienna, Austria

27. ANALYSIS OF MICRONUCLEI BY FLOW SORTING AND FISH
M. Nüsse1, A. Slavotinek2, B.M. Miller3
1 GSF-AG Durchflußzytometrie, D-85758 Oberschleißheim 2 Dept. Clinical Genetics, Churchill Hospital, Oxford 3PRPT. Preclinical Division, Hoffmann La-Roche AG, Basel

28. RESULTS OF THE COLLABORATIVE STUDY 1995 ON STANDARDIZATION AND QUALITY ASSESSMENT OF DNA FLOW CYTOMETRY
Friedrich J. Otto
Fachklinik Hornheide, D-48157 Münster

29. CYTOMETRIC ANALYSIS AND ISOLATION OF TRANSFECTED CELLS BY SURFACE EXPRESSED SELECTION MARKERS
K. Petry, R. Christine, G. Siebenkotten and A. Radbruch
Institute for Genetics ,University of Cologne, Weyertal 121, D-50931 Cologne, Germany

30. SUBCELLULAR LOCALIZATION STUDIES WITH NOVEL MITOCHONDRIAL STAINS
Martin Poot, Ananda G. Lugade, Jennifer Krämer, K. Sam Wells, Laurie J. Jones, Lisa Gibson, Stephen T. Yue, David Hanzel$, Victoria L. Singer and Richard P. Haugland
Molecular Probes, Inc., P.O. Box 22010 Eugene, OR 97402, and $Molecular Dynamics, Sunnyvale, CA 94086, U.S.A.

31. CALIBRATING A FLOW CYTOMETER WITH FLUORESCENT LATEX BEADS
Martin Poot, Sharon M. Swan, and K Sam Wells
Molecular Probes, Inc., Eugene, OR 97402, U.S.A.

32. EXTERNAL QUALITY CONTROL SURVEY OF IMMUNOPHENOTYPING OF THE CENTRAL REFERENCE INSTITUTION IN GERMANY ISSUES OF SPECIMENS AND INTERLABORATORY VARIABILITY
W. Prohaska, R. Kruse, W.-J. Geilenkeuser, T. Nebe, A. Radbruch, H. Diem, A. von Rücker
Working group of quality assessment in flow cytometry of the German Society of Clinical Chemistry

33. ANALYSIS AND ISOLATION OF RARE CELLS
Diether Recktenwald1, Hans Joachim Gross2, Stefan Miltenyi3
1 AmCell Corp., Sunnyvale, Ca, USA 2 Labor MittererstraBe, München 3 Miltenyi Biotec, Bergisch Gladbach

34. HIGH RESOLUTION FLOW CYTOMETRY AND P53 IMMUNOREACTIVITY IN STAGE I BORDERLINE EPITHELIAL OVARIAN TUMORS
Reich O., Pürstner P., Schöll W.
Department of Obstetrics and Gynecology, University of Graz, Auenbruggerplatz 14, 8010 Graz, Austria

35. QUANTIFICATION OF THE TOTAL CELLULAR CONTENT OF THE MYELOMONOCYTIC PROTEINS MRP8 AND MRP14 AND OF THEIR HETERODIMER RECOGNISED BY THE ANTIBODY 27E10 IN HUMAN MONOSYTES AND GRANULOCYTES USING DUAL LABEL FLOW CYTOMETRY
O. Röhrig, C.L. Klein, T. van Kooten C.J. Kirkpatrick
Institute of Pathology, Universityhospital of Mainz, Germany

36. CHARACTERIZATION OF PERIPHERAL BLOOD MONONUCLEAR PHAGOCYTE SUBPOPULATIONS
G. Rothe, J. Stöhr, G. Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg

37. A NEW FLOW CYTOMETRIC ASSAY FOR THE ANALYSIS OF THE HUMAN LYSOSOMAL ACID LIPASE
G. Schindler, G. Rothe, G. Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany

38. QUALITY CONTROL AND STANDARDIZATION OF FLOW CYTOMETRIC PLATELET ANALYSIS
G. Schmitz, S. Barlage, G. Rothe
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, F.R.G.

39. COMPARISON OF FLOWCYTOMETRIC DNA-MEASUREMENT WITH MORPHOMETRIC IMAGE ANALYSIS PARAMETER IN SELECTED OVARIAN CARCINOMA
Scholl W., Reich O., Pürstner P., Taucher A.
Department of Obstetrics and Gynecology, Univ. of Graz, Austria

40. QUALITY CONTROL OF IMMUNEPHENOTYPING IN AUSTRIA
E. Schönwald*, R. Jilch**
** Central Laboratory ( Univ.Prof. Dr. M. Fischer ) and * Department of Dermatology ( Univ. Prof. Dr. F. Gschnait ) Municipal Hospital Lainz, Vienna, Austria

41. SOURCES OF ERRORS IN QUANTITAVE IMAGE ACQUISITION IN CYTOMETRY
Schwarzmann P., Mähner G.
Inst. für Physikallshe Elektronik, Univ. Stuttgart,FRG

42. FLOW CYTOMETRIC DETECTION OF INTRACELLULAR PLATELET ANTIGENS
Peter Sedlmayr1, Barbara Grosshaupt1, 2, Wolfgang Muntean2, Astrid Blaschitz3, Gottfried Dohr3
Departments of Internal Medicine1 and Pediatrics2, and Institute for Histology and Embryology3, Karl-Franzens-University Graz, Austria

43. CYTOMETRIC EVALUATION OF RUSH BEE-VENOM IMMUNOTHERAPY
J.A. Segura, J. Irsch*, A. Löhndorf*, N. Hünzelmann* and A. Radbruch Institute for Genetic. University of Cologne. Weyertal 121. 50931 Cologne, Germany.*Clinic of Dermatology of the University of Cologne. Joseph-Stelzmann Str. 9.50931 Cologne, Germany.

44. 5-AMINOLEVULINIC ACID UPTAKE, SYNTHESIS OF PROTOPORPHYRIN IX, AND EFFLUX OF PORPHYRINS IN TWO UROTHELIAL CARCINOMA CELL LINES
P. Steinbach1, H. Weingandt1, R. Baumgartner2, M. Kriegmair2, F. Hofstädter1, R. Knüchel1
Institute of Pathology, University of Regensburg and 2Dept. of Urology, University of Munich, Germany

45. NON-RADIOACTIVE LIFE SPAN MEASUREMENTS OF RED BLOOD CELLS BY FLOW CYTOMETRY AND ITS COMPARISON WITH RADIOACTIVE LABELING
Trastl. C.1; Beisker. W.2; Berg D.3; Hoffmann-Fezer, G.1
(1): GSF - Institut fur Immunologie, (2): GSF - Arbeitsgruppe Durchflusszytometrie, (3): GSF - Institut für Strahlenbiologie, Ingolstädter Landstr. 1,D-85764 Oberschleissheim, Germany

46. SENSITIVITY OF MAMMALIAN SPERMATOGENIC CELLS TO IONIZING IRRADIATION
I. Tatchen, W. Göhde
Insititut für Strahlenbiologie, Westfälische Wilhelms-Universität, Münster, Germany

47. COMPUTER CLASSIFICATION OF FLOW CYTOMETRIC THREE COLOUR IMMUNEPHENOTYPES FROM BLOOD LEUKOCYTES AND BONE MARROW OF LOW AND HIGHGRADE NON-HODGKIN-LYMPHOMA AND CHRONIC LYMPHATIC LEUKEMIA PATIENTS
G.Valet1, G.Schmidtke2, U.Schmücker2, G.Brittinger2, H.G.Höffkes2
1) Max-Planck-lnst.f.Biochemie, 82152 Martinsried, 2) Abt.Hämatologie, Medizinische Univ.Klinik, 45122 Essen, Germany

48. EXCIMER FLUORESCENCE COMPARED TO DEPOLARIZATION IN THE ANALYSIS OF HYDROPHOBIC MEMBRANE DOMAINS
M. Wimmer, G. Rothe, G. Schmitz
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany

=== Poster Presentations ===
49. CGH IMAGING BY A ONE CHIP TRUE COLOR CCD CAMERA
Harald Bornfleth1, Klaus Aldinger1, Michael Hausmann1, Anna Jauch2 and Christoph Cremer1,3
1= Institute of Applied Physics, 2=lnstitute of Human Genetics and Anthropology, 3=lnterdisciplinary Centre of Scientific Computing (IWR), University of Heidelberg, Heidelberg, F.R. Germany

50. SIMULTANEOUS REGISTRATION OF CALCIUM TRANSIENTS AND REACTIVE OXYGEN INTERMEDIATES OF HUMAN POLYMORPHONUCLEAR GRANULOCYTES IN RESPONSE TO ENVOIRONMENTAL AGENTS
W. Grundler1, P. Dirscherl2, K Marx1, I. Beck-Speier2, W. Beisker1, K. Maier2, and A. Stampfl3
GSF-Forschungszentrum fiir Umwelt und Gesundheit, AG Durchflußzytometrie, Institut für Inhalationsbiologie2 und Institut fur Toxikologie3, D-85758 Oberschleißheim

51. THE HEIDELBERG SLIT-SCAN FLOW FLUOROMETER
Michael Hausmann1, Alexander Rohrbach1, Dietmar Wolf1, Klaus Aldinger1, Michael Schurwanz1, Jürgen Dölle2, Arnulf Keese3, Martin Crone1, Christoph Cremer1
1= Institute of Applied Physics, University of Heidelberg, D-69120 Heidelberg, Germany (present addresses: 2=lBM Development Laboratory, D-71032 Böblingen, FRG; 3= Bertelsmann Development, D-33311 Gütersloh)

52. FLOW-CYTOMETRIC CHARACTERIZATION OF BATCH CULTIVATED BACTERIA WITH FLUORESCENT, rRNA-TARGETED OLOGONUCLEOTID PROBES
Carsten Herrmann, Andreas Lösche, Thomas Bley
Projektgruppe Biosignale, Abteilung Biotechnologie, Fakultät für Biowissenschaften,Pharmazie und Psychologie, Universität Leipzig, Permoserstr. 15, 04138 Leipzig.

53. ANTI-P53 AUTOANTIBODIES IN SERA OF PATIENTS WITH ORAL SQUAMOUS CELL CARCINOMA
Ch. Hofele*, M. Volkmann+, M. Schmitt*, W. Fiehn+, J. Zöller*. J. Mühling*
(*Dept. of Oral and Maxillofacial Surgery, University of Heidelberg, INF 400, D-69120 Heidelberg, Germany, +Central Laboratory, Medical University Hospital, Bergheimer Str. 58, D-69115 Heidelberg, Germany)

54. CELL CYCLE AND CELL SIZE ANALYSIS OF DIFFERENT SACCAROMYCES YEASTS
K.-J. Hutter1, J.Schärfe2
1 DKFZ Heidelberg, Im Neuenheimer Feld 280, R.Süssmuth, Institut f. Mikrobiologie, Univ. Hohenheim, 2 Schärfe System, Reutlingen

55. TIME SHIFTING OF CIRCADIAN PATTERNS OF LYMPHOCYTE SUBSETS AND EXPRESSION OF CD2, CD11a AND CD44 IN HUMAN PERIPHERAL BLOOD
T.O.Kleine1, R. Hackler1, P. Z&oouml;fel2, J. Albrecht3
1 Med. Zentrum für Nervenheilkunde, Funktionsbereich Neuro- chemie, Universität Marburg a.d. Lahn, 2 Hochschulrechenzen- trum der Universität Marburg, 3 Becton Dickinson, Heidelberg

56. INFLUENCE OF TOTAL SLEEP DEPRIVATION ON CIRCADIAN VARIATIONS OF LYMPHOCYTE SUBSETS IN HUMAN PERIPHERAL BLOOD FROM HEALTHY MALE VOLUNTEERS
T.O. Kleine1, W. Schreiber2, R. Hackler1, P. Zöfel2, J.Albrecht3
1 Med. Zentrum für Nervenheilkunde, Funktionsbereich Neurochemie, Universität Marburg a.d. Lahn; 2 Klinik für Psychartrie im Med. Zentrum für Nervenheilkunde, Universität Marburg a.d. Lahn; 2 Hochschulrechenzentrum der Universität Marburg, 3 Becton Dickinson, Heidelberg.

57. DNA IMAGE CYTOMETRY ON SECTIONS: MEASUREMENT OF ENTIRE OR CUT NUCLEI?
T.KIapperstück and W. Wohlrab
Department of Dermatology, Martin Luther University Halle-Wittenberg, Germany

58. FAST KINETICS MEASUREMENT OF INTRACELLULAR Ca2+ ON THE FACScan
Ladusch, M., Jüngel, A., Drössler, K.
University Leipzig, Institute of Zoology, Talstrasse 33, D-04301 Leipzig

59. FLOWCYTOMETRIC TYPING OF PREADIPOCYTES IN MUSCLE TISSUE
Bertold Löhrke, Torsten Viergutz, Jochen Wegner, Klaus Ender
Research Institute of Animal Biology, Dummerstorf-Rostock

60. FLOW CYTOMETRY ANALYSIS USING ACTIVATED MICROBEADS
Reinhard Malsch, Lukas Piazolo, Daniel Melui and Job Harenberg
First Dept. of Medicine, Faculty of Clinical Medicine Mannheim, University of Heidelberg, Theodor Kutzer Ufer, 68167 Mannheim, Germany.

61. STANDARDIZATION OF PROTAMINE-COATED MICROBEADS USING FLOW CYTOMETRY FOR HEPARIN QUANTITATION
Daniel Melui, Reinhard Malsch, Lukas Piazolo and Job Harenberg
First Dept. of Medicine, Faculty of Clinical Medicine Mannheim, University of Heidelberg, Theodor-Kutzer-Ufer, 68167 Mannheim, Germany

62. DETERMINATION OF BACTERIAL VIABILITY WITH A NOVEL 488 NM-EXCITABLE NUCLEIC ACID STAIN
Paul J. Millard, Bruce Roth, Stephen T. Yue, Laurie J. Jones, and Martin Poot
Molecular Probes, Inc., Eugene, Oregon 97402, U.S.A.

63. FLOW CYTOMETRIC DETECTION OF APOPTOSIS IN RAT T CELL LINES
E.Mix1, U.K.Zettl1,2, J.Zielasek2, M.Stangel3, K.V.Toyka2, H.-P.Hartung2, R.Gold2
1Dept. of Neurology, Hosp. Nerv. Dis.,Universität Rostock, 2Dept. of Neurology, Universität Würzburg, 3Dept. of Neurology, Freie Universität Berlin, Germany

64. DNA DISTRIBUTIONS OF SOME BACTERIA: INFLUENCE OF GROWTH PHASE, GROWTH RATE AND NUTRIENTS
Susann Müller, Andreas Lösche and Thomas Bley
Projektgruppe Biosignale, Abteilung Biotechnologie, Fakultät für Biowissenschaften, Pharmazie und Psychologie, Universität Leipzig, Permoserstr.15, D-04318 Leipzig

65. FLOW CYTOMETRIC ANALYSIS OF THE PHYSICAL ASSOCIATION BETWEEN SURFACE RECEPTOR AND CYTOSKELETON
B. Nebe, J. Rychly
Klinik für Innere Medizin, Universität Rostock

66. EVALUATION OF FOUR MONOCLONAL ANTIBODIES AGAINST HLA-B27 FOR THEIR RELIABILITY IN HLA-B27 TYPING WITH FLOW CYTOMETRY (FC). COMPARISON WITH THE CLASSIC MICROLYMPHOCYTOTOXIC TEST (MLCT)
J. Neumüller, D.W.M. Schwartz**, E. Dauber**, W.R. Mayr**
Ludwig Boltzmann Institute for Rheumatology and Balneology, Vienna, Austria, *CIinical Department for Blood Group Serology, University of Vienna, Vienna, Austria

67. DETECTION OF RED FLUORESCENT MICROPHERES USING AN ALL- SOLID-STATE FLOW CYTOMETER
Stefan Niehren1, Wolfgang Kinzelbach1, Stefan Seeger2, Jürgen Wolfrum2
1 Institute of Environmental Physics, University of Heidelberg, Im Neuenheimer Feld 366, 69120 Heidelberg 2 Institute of Physical Chemistry, University of Heidelberg, Im Neuenheimer Feld 253, 69120 Heidelberg

68. FLOW CYTOMETRIC ANALYSIS OF T CELL REPERTOIRE: A RAPID METHOD FOR PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST THE HUMAN a/beta T CELL RECEPTOR
C. Otto, F. Struck, K. Littmann-JanGen, J. Collins, A. Lingnau, K.E.J. Dittmar
Gesellschaft fur Biotechnologische Forschung (GBF), Dept. of Applied Genetics, D-38124 Braunschweig, FRG

69. BINDING TO LEUKOCYTES AND INTACT BIOLOGICAL ACTIVITY OF A FLUORESCENT LABELED LMM-HEPARIN
L. Piazolo, J. Harenberg, R. Malsch and D. L. Heene
1st. Department of Medicine, Medical University Clinic, Theodor-Kutzer Ufer, 68167 Mannheim, Germany

70. RESOLUTION EFFECTS IN "PRACTICAL" LIGHT MICROSCOPY AND THE APPLICATION OF A 2pi TILTING DEVICE
Bernd Rinke, Joachim Bradl, Bernhard Schneider, Michael Hausmann, Christoph Cremer
Institute of Applied Physics, University of Heidelberg, D-69120 Heidelberg, Germany

71. CELL CYCLE OR PROLIFERATION DEPENDANCY OF DIFFERENTIAL EXPRESSION OF CD34 BY HUMAN ENDOTHELIAL CELLS?
O. Röhrig, C.L. Klein, T. van Kooten, F. Bittinger, C.J.Kirkpatrick
Institute of Pathology, University of Mainz, Germany

72. FLOW CYTOMETRIC DNA ANALYSIS OF PROSTATE TUMORS
K. Romanakis1, B. Wullich2, K. Becker3, B. Kopper3, and H. Zankl1
1University of Kaiserslautern, Dept. Human Biology and Human Genetics, 67663 Kaiserslautern, 2University of the saar, Dept. of Urology, 68141 Homburg/Saar, 3Universitv Hospital of Kaiserslautern, 67655 Kaiserslautern

73. DNA-CYTOMETRY AND KARYOMETRIE OF CERVICAL INTRAEPITHELIAL NEOPLASIAS
M. Ruhnke, A. Coumbos W. Kühn
Department Gynecol. Obstet., Klinikum Benjamin Franklin, Free University of Berlin, Germany

74. EXPRESSION OF ADHESION MOLECULES OF THE IMMUNOGLOBULIN SUPERFAMILY ON HUMAN RENAL CARCINOMA CELL LINES.
Schardt, C., Gerharz, CD., Gabbert, HE.
Institute of Pathology, Düsseldorf, Germany

75. MORPHOMETRIC AND DENSITOMETRIC IMAGE ANALYSIS ON BLADDER CARCINOMA CELLS STAINED WITH FLUORESCENT DYES
Wolfgang Schildbach, Elmar Endl, Pia Steinbach, Gabriele Fauser, Josef R.Aschoff and Ferdinand Hofstädter
Institute of Pathology, University of Regensburg,D-93042 Regensburg, Germany

76. ENDOMITOTIC CHROMOSOMES IN THE NURSE CELLS OF CHRYSOMYA RUFIFACIES
Michael Schöttke
Zoologisches Institut der Universität Kiel, Olshausenstr. 40, D-24098 Kiel

77. TELEMICROSCOPY - A TOOL FOR THE REMOTE EVALUATION OF CYTOLOGICAL MATERIAL
Schwarzmann P., Schmid J., Schnörr C., Binder B.
Inst. für Physikalische Elektronik, University Stuttgart,FRG

78. FLOW CYTOMETRIC ANALYSIS OF CELL CYCLE PERTURBATIONS AND MlCRONUCLEI AFTER UV B- AND gamma-IRRADIATION
Weller E.M.1, Hain J.1, Jung T.1, Kinder R.2, Köfferlein M.3, Burkart W.1 and Nüsse M.2
1BfS-lnst. f. Strahlenhygiene, 2GSF-AG Durchflusszytometrie, 3 GSF Inst. f. Biochemische Pflanzenpathologie, 85762 Oberschleiss- heim, Germany

79. FLOW SORTING OF BACTERIA FOR PCR-ANALYSIS
G. Wallner1, S. Spring2, B. Fuchs2, W. Beisker1, and R. Amann2
1 GSF-Forschungszentrum, AG Durchflußzytometrie, D-85764 Oberschleißheim 2 TU München. Lehrstuhl fur Mikrobiologie, D-80290 München

80. OXIDATIVE STRESS AND NO* INDUCE APOPTOSIS IN RAT MYOGENIC CELLS
U.K.Zettl1,2, E.Mix1, J.Zielasek2, M.Stangel3, H.Meyer- Rienecker1, K.V.Toyka2, H.-P.Hartung2, R.Gold2
1Dept. of Neurology, Hosp. Nerv. Dis., Universität Rostock, 2Dept. of Neurology, Universitat Würzburg, 3Dept. of Neurology, Freie Universität Berlin, Germany

DGZ