Deutsche Gesellschaft für Zytometrie
(DGZ)


Abstract titles of Annual Meeting
Heidelberg, Oct.20-22, 1994

Analytical Cellular Pathology 8,395-421 1995

=== KEY NOTE LECTURES ===

1. MULTIWAVELENGTH 3D MAGING OF IMMUNO- LOGICAL AND GENETICAL MARKERS IN THE CELL NUCLEUS BY CONFOCAL MICROSCOPY AND IMAGE RECONSTRUCTION
M.Robert-Nicoud
Dynamique de l'Organisation du Genome, "DyOGen", Institut Albert Bonniot, Universite Joseph Fourier, 38706 La Tronche Cedex, France.

2. 3D RECONSTRUCTION AND IMAGE ANALYSIS TECHNIQUES FOR CONFOCAL LASER SCANNING MICROSCOPY
Y.Usson and M.Robert-Nicoud
DyOGen, Institut Albert Bonniot, Univ. Joseph Fourier, Grenoble, France

3. HUMAN B CELL MALIGNANCIES: CYTOMETRIC ANALYSIS IN THE CONTEXT OF NORMAL B CELL DIFFERENTIATION
Max D. Cooper
University of Alabama at Birmingham and the Howard Hughes Medical Institute, Birmingham, AL 35294, USA

=== ORAL PRESENTATIONS ===

1. IMMUNE PHENOTYPIC PARAMETERS AS BASIS FOR A HIV- CLASSIFICATION CORRESPOND WITH THE NATURAL COURSE OF THE DISEASE R.Bauer1, R.Böhm1, Winkler2, H.Kreuter2
1Department of Dermatology, University of Bonn and 2Wissenschaftliches Institut der Aerzte Deutschlands (WIAD e.V.)

2. MONITORING OF CELL STATE DEPENDENT PERFORMANCE OF MICROBIAL POPULATIONS IN BIOREACTORS
Th.Bley, S.Müller, A.Lösche
University of Leipzig, Department of Biotechnology, D-04318 Leipzig

3. HIV-RELATED KAPOSI SARCOMA (KS): IMMUNOBIOLOGICAL EFFECTS OF INTERFERON-a (IFNa) AS DETECTED BY FLOW CYTOMETRIC ANALYSIS
I.Böhm, R.Bauer
Department of Dermatology, University of Bonn, Germany

4. EXPERIMENTAL EVIDENCE THAT METHOTREXATE BLOCKS THE BINDING OF INTERLEUKIN 1b TO THE INTERLEUKIN 1 RECEPTOR ON TARGET CELLS
M.Brody, I.Böhm, R.Bauer Department of Dermatology, University of Bonn, Germany

5. DNA CONTENT IN COMPARISON WITH INTRAOPERATIVE PLEURAL LAVAGE AS A PROGNOSTIC FACTOR IN LUNG CANCER
J.Buhr1, R.Deisz1, K.H.Berghäuser2, C.Kelm1, T.Zimmermann1, T.Dreyer2
1Dept. of Surgery and 2Institute of Pathology of Justus Liebig University Giessen

6. SUPERRESOLUTION FAR FIELD LIGHT MICROSCOPY: ITS POTENTIAL FOR THE ELUCIDATION OF NUCLEAR STRUCTURE
C. Cremer
Institute of Applied Physics, University of Heidelberg, D-69120 Heidelberg

7. COMPARATIVE ANALYSIS OF CELL DAMAGE BY POLAR DYE EXCLUSION OR ELECTRICAL CELL VOLUME DETERMINATION
Elmar Endl, Pia Steinbach, Jürgen Schärfe, Sonja Fickweiler, Klaus Wörle, Ferdinand Hofstädter
Institut für Pathologie, Universität Regensburg, D-93042 Regensburg, Schärfe System, D-72760 Reutlingen, Germany

8. COMPARISON OF CELL KINETIC PARAMETERS IN TUMORS OF THE UPPER GASTROINTESTINAL TRACT
K.P. Gilbertz, K.-P. *Sendler, A. *Fink, U. & D. van Beuningen
Institut fur Radiobiologie, Akademie des Sanitäts- und Gesundheitswesens der Bundeswehr, München; *Chirurgische Klinik und Poliklinik, Technische Universität München

9. FLOW CYTOMETRIC ANALYSIS OF S-PHASE FRACTION AND PLOIDY IN NODE-NEGATIVE BREAST CANCER
N.Harbeck, E.Schüren, P.Ziffer, L.Pache, M.Schmitt, Ch.Thomssen, F.JAenicke, P. Dettmar*, W.Nathrath* and H.Graeff
Frauenklinik and *Institut für Pathologie, Technische Universität, München.

10. PHARMACOKINETIC ANALYSIS OF HEPARIN USING PROTAMINE-COATED LATEX PARTICLES AND FLOW (CYTOMETRY
J.Harenberg, L.Piazolo, R.Malsch, D.L.Heene
1st Department of Medicine, Faculty of Clinical Medicine Mannheim, University of Heidelberg, D-68167 Mannheim, Germany

11. ANALYSIS OF HEPARIN BINDING TO HUMAN LEUKOCYTES USING A FLUORESCEIN-5-ISOTHIOCYANATE LABELED HEPARIN FRAGMENT
J.Harenberg, R.Malsch, L.Piazolo, D.L. Heene
1st Department of Medicine, Faculty of Clinical Medicine Mannheim, University of Heidelberg, D-68167 Mannheim, Germany

12. CIMS (CONTINUOUS IMMUNO MAGNETIC SORTER) A POWERFUL TECHNIQUE FOR SORTING OF BIOLOGICAL PARTICLES
R.Hartig, M.Hausmann, M.Kraus, C.Cremer
Institute of Applied Physiol, University of Heidelberg, D-69120 Heidelberg, Germany

13. KARYOTYPIC PROGRESSION AND PROGNOSIS IN ORAL CARCINOMA
Hemmer J, Bartkowiak B
University of Ulm, Division of Tumorbiology, D-89070 Ulm, Germany

14. SEMIQUANTITATIVE MORPHOLOGY, IMMUNOHISTOCHEMISTRY, AND FLOW CYTOMETRY OF HUMAN SPERMATOGENESIS
A.Hittmair, H.Rogatsch, H.Feichtinger, A.Hobisch*, R.Bale, G.Mikuz
Departments of Pathology and *Urology, University of Innsbruck, Austria

15. LIPOSOME MEDIATED UPTAKE OF ANTISENSE OLIGONUCLEOTIDES BY CANCER CELLS AS STUDIED BY FLOW CYTOMETRY AND LASER SCANNING MICROSCOPY
S. Höhl, O.Wilhelm, E.Schüren, M.Schmitt, H.Graeff
Frauenklinik der Technischen Universität München

16. COMBINED FLOW CYTOMETRIC IMAGING AND SORTING IN A MACRO FLOW SYSTEM FOR AQUATIC APPLICATIONS
M.Höreth, W.Runge, E.Gloáner, K.Enzensberger, C.Steiner, V.Kachel
Max-PIanck-lnstitut für Biochemie, D-82152 Martinsried, Germany

17. SORTING AND ANALYSIS OF LYMPHOCYTES INVOLVED IN HUMORAL ALLERGIC RESPONSES
J.Irsch, E.Maggi*, A.Ruffilli$, A.Radbruch
Institute for Genetics, University Cologne, D-50931 Cologne, Germany, *Institute for Clinical Immunology, I-50134 Florence, $Int. Institute of Genetics, I-80125 Naples, Italy

18. CELL-CELL CONTACTS OF THE ADHERING TYPE: ULTRASTRUCTURE AND MOLECULAR COMPOSITION
J.Kartenbeck
Division of Cell Biology, German Cancer Research Center, Heidelberg, Germany

19. RADIOSENSITIVITY OF SIGNAL TRANSDUCTION PATHWAYS LEADING TO ACTIVATION OF THE NADPH OXIDASE
W.Kaffenberger, K.-P.Gilbertz, D. van Beuningen
Institute of Radiobiology, FAF Med.Acad., Munich, Germany

20. DIOXIN (2,3,7,8-TETRACHLORODIBENZO-p-DIOXIN) INDUCED SKEWING OF THYMOCYTE DIFFERENTIATION: ANALYSIS OF EXPRESSION OF ADHESION MOLECULES
J.Kremer, Ch.Esser
Medical Institute of Environmental Hygiene, Auf'm Hennekamp 50, 40225 Düsseldorf

21. IMPROVEMENT OF BLADDER TUMOR IDENTIFICATION BY COMBINED FLOW CYTOMETRIC MEASUREMENT OF DNA ANEUPLOIDY AND DISTRIBUTION OF 486 P ANTIGEN
H.Leyh, A.Lehmer, R.Hartung
Department of Urology, Technische Univ. München

22. DNA-PLOIDY PATTERNS IN BENIGN PROSTATIC HYPERPLASIA*
H.Leyh, A.Lehmer, R.Hartung
Department of Urology, Technische Univ. München

23. PLOIDY PATTERNS IN RENAL CELL CARCINOMA
H.Leyh, A.Lehmer, R.Hartung
Department of Urology, Technische Univ. München

24. CONFOCAL THETA MICROSCOPY AND 4Pi-CONFOCAL THETA MICROSCOPY
S.Lindek, E.H.K.Stelzer
European Molecular Biology Laboratory (EMBL), Light Microscopy Group, D-69012 Heidelberg, Germany

25. PLOIDY PATTERNS IN RENAL CELL CARCINOMA
H.Leyh, A.Lehmer, R.Hartung
Department of Urology, Technische Univ. München

26. SEPARATION OF MESOPHYLL AND BUNDLE-SHEATH CHLOROPLASTS FROM PLANTS OF PHOTOSYNTHETIC C4 TYPE BY FLOW CYTOMETRY
A.Meister, E.Pfündel
Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany

27. POSSIBILITIES AND LIMITS OF FEULGEN CYTOPHOTOMETRY IN PARAFFIN-EMBEDDED TISSUE
W. Mellin
Institute of Pathology, University of Magdeburg

28. PROGNOSTIC IMPACT OF PROLIFERATION IN COMPLETELY RESECTED ADENOCARCINOMA OF THE ESOPHAGUS
H.Nekarda1, T.Nakamura3, K.Becker2, A.Hölscher1, J.R.Siewert1
1Chirurgische Klinik and 2Inst.f. Pathologie der TU, D-81675 München, 3Tokyo Women's Medical College, Tokyo, Japan

29. PAS IIIi - A FLOW CYTOMETER FOR ROUTINE IMMUNOLOGY
V.0st
Partec GmbH, D-48161 Münster

30. RESULTS OF THE COLLABORATIVE STUDY 1994 ON FLOW CYTOMETRIC DNA MEASUREMENTS
F.J. Otto
Fachklinik Hornheide, D-48157 Münster

31. ANALYSIS OF THE BINDING OF HEPARIN ON PROTAMINE COATED LATEX PARTICLES USING THE FLOW CYTOMETRY
L.Piazolo, J.Harenberg. R.Malsch, D.L.Heene
1st Department of Medicine, Faculty of Clinical Medicine Mannheim, University of Heidelberg, D-68167 Mannheim, Germany

32. MORPHOMETRY ON CERVICAL SMEARS
M. Ruhnke and W. Kühn
Dept. Gynecol. Obstet., Klinikum Benjamin Franklin, Free University of Berlin, Germany

33. ENHANCING FLUORESCENCE INTENSITY AND DETECTION OF RARE EPITOPES WITH MAGNETOFLUORESCENT LIPOSOMES
A.Scheffold, S.Miltenyi*, A.Radbruch
Institute for Genetics, University of Cologne, 50931 Cologne, *Miltenyi Biotec GmbH, 51429 Bergisch Gladbach, Germany

34. ANALYSIS OF THE BINDING OF HEPARIN ON PROTAMINE COATED LATEX PARTICLES USING THE FLOW CYTOMETRY
L.Piazolo, J.Harenberg. R.Malsch, D.L.Heene
1st Department of Medicine, Faculty of Clinical Medicine Mannheim, University of Heidelberg, D-68167 Mannheim, Germany

35. QUALITY CONTROL IN FLOW CYTOMETRY THROUGH MULTIPARAMETER DATA ANALYSIS
E.Smet
Becton Dickinson Immunocytometry Systems Europe, Erembodegem, Belgium

36. INVASION ANALYSIS OF LUNG TUMOR CELLS IN GEL MATRICES
Strohmaier A.-R., Spring H., Spiess E.
Biomedizinische Strukturforschung, Deutsches Krebsforschungszen- trum, Heidelberg

37. MONOCLONAL ANTIBODIES AGAINST HUMAN CD34+ CELLS ISOLATED FROM PERIPHERAL BLOOD
A.Thiel, A.Radbruch, G. von Hesberg, S. Milteny*
Institute for Genetics, University of Cologne, 50931 Cologne, *Miltenyi Biotec GmbH, 51429 Bergisch Gladbach, Germany

38. QUANTITATIVE ANALYSIS OF ACTIVE TRANSCRIPTION SITES WITHIN INTACT NUCLEOLI: A LASER SCAN MICROSCOPIC ANALYSIS
M.F.Trendelenburg, O.V.Zatsepina*, H.Spring
Biomedical Structure Analysis Group, German Cancer Research Center (DKFZ), Germany *A.N. Belozersky Institute, Moscow State University, Russia

39. QUANTIFICATON OF INTRACELLULAR CATHEPSIN ACTIVITIES IN HUMAN LUNG TUMOR CELLS BY FLOW CYTOMETRY
B.Ulbricht1, E.Spiess1, W.Ebert2
1DKFZ, AG Biomedizinische Strukturanalye, 69120 Heidelberg, 2Thoraxklinik der LVA Baden, 69126 Heidelberg, Germany

40. CHARACTERIZATION AND SIGNAL TRANSDUCTION OF THE HEAD ACTIVATOR RECEPTOR IN NEURAL CELL LINE NH15-CA2
H.Ulrich, A.Tarnok
ZMNH, Center for Molecular Neurobioloay, UKE, 20246 Hamburg, Germany

41. CRITERIA FOR HIGH-RESOLUTION DNA FLOW CYTOMETRY
W.Ulrich
Flow Cytometric Service Center, D-72667 Schlaitdorf

42. DIFFERENT PROGNOSTIC IMPACT OF INVASION-ASSOCIATED PROTEASES, PLOIDY AND S-PHASE FRACTION IN COLORECTAL CANCER PATIENTS
H.Vogelsang, H.Nekarda, W.Schröder, F.Huber, J.R.Siewert
Chirurgische Klinik rechts der Isar der TU München, D-81675 München

43. IN VITRO FLUORESCENCE ANALYSIS OF THE ENDOGENOUS PHOTOSENSITIZER PROTOPORPHYRIN IX
H.Weingandt1, P.Steinbach1, R.Baumgartner2, M.Kriegmair2, F.Hofstädter1, R.Knüchel1
1Institut für Pathologie der Universität Regensburg, D-93042 Regensburg, 2Urologische Klinik und Poliklinik der Universität München, D- D-81366 München

44. DESIGN CRITERIA FOR FLOW CYTOMETRIC IMAGING
J. Wietzorrek, R. Kühn, M. Stadler, V. Kachel
Max-Planck-Institut für Biochemie, D-82152 Martinsried, Germany

45. LOW CYTOMETRIC ANALYSIS OF THE EPIDERMAL GROWTH FACTOR RECEPTOR (EGF-R) ON HUMAN OVARIAN CARCINOMA CELLS
C.Will, A.Schäfer, E.Schüren, S.Schwarze, M.Schmitt, O.Wilhelm, V.Möbus, H. Graeff
Frauenklinik der TU München and Frauenklinik der Universität Ulm.

=== POSTERS ===

1. FLOW CYTOMETRIC EVALUATION OF CALCIUM TRANSIENTS IN HUMAN POLYMORPHNUCLEAR GRANULOCYTES APPLYING A NOVEL APPROACH TO DATA ANALYSIS
P.Dirscherl1, I.Beck-Speier1, W Beisker2, W.Grundler2, K.Maier1, A.Stampfl3
1GSF, Projekt Inhalation, 2Departement for Cytometry and 3Institute for Toxicology, D-85764 Neuherberg, Germany

2. THE INFLUENCE OF ALCOHOL, TOBACCO CONDENSATE AND SALIVARY GLAND FUNCTION ON THE ORAL MUCOSA OF THE RAT
Ch.Hofele1, J.Zöller1, H.Maier2, J.A.Born3
1University Hospital of Maxillo-Facial Surgery, 2University Hospital of Oto-Rhino-Laryngology 3University Institute of Pathology, University of Heidelberg, D-69120 Heidelberg

3. IDENTIFICATION OF OXYTOCIN RECEPTORS ON BOVINE LUTEAL CELLS
J.Kotwica1, T.Viergutz2, R.Pöhland2, W.Kanitz2
1Polish Academy of Science Olsztyn, Division of Reproductive Endocrinology and Pathology, Poland, 2Research Institute for the Biology of Farm Animals, Department of Reproduction, Dummerstorf, Germany

4. CIRCADIAN VARIATIONS OF LYMPHOCYTE SUBSETS AND EXPRESSION OF CD44, CD11a AND CD2 IN HUMAN PERIPHERAL BLOOD
T.O.Kleine1, R.Hackler1, P.Zofel2, J.Albrecht3
1 Med. Zentrum fur Nervenheilkunde, Funktionsbereich Neurochemie, Universitat Marburg a.d. Lahn, 2 Hochschulrechenzentrum der Universität Marburg, 3Becton Dickinson, Heidelberg.

5. THE OXIDATIVE AND MEMBRANE POTENTIAL RESPONSE OF PBMC TO TRH
B.Löhrke, M.Derno, W.Jentsch, H.-D.Matthes, G.Dietl
Research Institute of Animal Biology, D-18196 Dummerstorf.

6. B CELL ACTIVATION IN THE CEREBROSPINAL FLUID(CSF): COMPARISON OF INTERLEUKIN 2 RECEPTOR EXPRESSION, INTRACELLULAR IMMUNOGLOBULINS AND OLIGOCLONAL BANDS
E.Mix1, R.Lehmitz2, T.O.Kleine3, R.Hackler3, H.MeyerRienecker1
1 Dept. of Neurology, Hosp. Nerv. Dis., 2 Central Laboratory of CSF Diagnostics, Med. Faculty, University of Rostock, 3 Division of Neurochemistry, Med. Center of Neurosci., University of Marburg, Germany

7. CELL CYCLE AND PHB-SYNTHESIS OF METHANOL UTILIZING BACTERIA UNDER LIMITING CONDITIONS
S.Müller, J.U.Ackermann, A.Lösche, W.Babel, Th.Bley
University of Leipzig, Department of Biotechnology, D-04318 Leipzig

8. SIGNAL TRANSDUCTION THROUGH INTEGRINS IN HEPATOCYTES
B.Nebe1, J.Rychly1, W.Bohn2
1 Department of Internal Medicine, Unversity of Rostock, 2 Heinrich-Pette-Institute at the University of Hamburg

9. FLOW CYTOMETRIC MEASUREMENTS OF SPONTANEOUS APOPTOTIC CELLS IN FRESH TUMOR SAMPLES
K.Romanakis1, A.Genzlinger1, P.K.Gadhia2, H.Zankl1
1 University of Kaiserslautern, Dept. Human Biology and Human Genetics, D-67663 Kaiserslautern, Germany, 2 Dept.Biosciences, South Gujarat University, Surat 395 007, India

10. DNA ANALYSIS BY FLOW CYTOMETRY OF UP TO NINE YEAR OLD METHANOL/ACETIC ACID FIXED CHILDHOOD ALL SAMLPES
K.Romanakis1, A.Argyriou-Tirita2, H.Zankl1, O.A.Haas2
1 University of Kaiserslautern, Dept. Human Biology and Human Genetics, D-67663 Kaiserslautern, Germany, 2 Children's Cancer Research Institute, St. Anna Children's Hospital, A-1090 Vienna. Austria

11. RAPID DENSITOMETRIC AND MORPHOMETRIC IMAGE ANALYSIS ON FROZEN SECTION BY SELECTIVE FLUOROCHROMIC NUCLEAR STAINING
W.Schöll, P.Pürstner, H.Auner
Dept. of Ob/Gyn, Univ. of Graz, Austria

12. HIGHER INDUCTION OF MICRONUCLEI BY MITOMYCIN C AND g-RAYS IN DIFFERENTIATED SOMATIC CELLS THAN IN UNDIFFERENTIATED EMBRYONIC CELLS OF THE MOUSE IN VITRO MEASURED BY FLOW CYTOMETRY
U.Sehlmeyer1, W.Beisker2, A.M.Wobus1
1 Institut für Pflanzengenetik und Kulturpflanzenforschung, D- 06466 Gatersleben, 2 GSF-lnstitut für Biophysikalische Strahlenforschung, D-85764 Oberschleißheim, Germany

13. THE EFFECT OF THE ANTITUMOR PLANT ALKALOID THALIBLASTIN ON THE CELL-CYCLE PROGRESSION OF OVARIAN TUMOR CELL LINES SENSITIVE AND RESISTANT TO CISPLATIN
F.Seifert1, K.-J.Hutter2, D.Todorov3, W.J Zeller1
1 Institute of Toxicology and Chemotherapy, and 2 Institute of Radiology and Pathophysiology, Applied Immunology, Flow Cytometry, 3 National Oncological Center, BG-1156 Sofia, Bulgaria

14. FLOW-CYTOMETRIC DNA ANALYSIS ON FINE-NEEDLE ASPIRATES FROM BREAST TUMORS
M.Us-Krasovec, A.Pogacnik, R.Golouh, B.Skrbinc, M.Bracko
Institute of Oncology, Ljubljana, Slovenia

15. FLOW CYTOMETRIC ANALYSIS OF ACTIVATED SLUDGE WITH rRNA- TARGETED OLIGONUCLEOTIDE PROBES
G.Wallner, R.Amann
GSF-Forschungszentrum für Umwelt und Gesundheit, AG Durchfluázytometrie, and TU München, Lehrstuhl für Mikrobiologie

16. FLOW CYTOMETRIC MEASUREMENT OF HYDROGEN PEROXIDE (H2O2) BURST IN CEREBROSPINAL FLUID (CSF) CELLS
U.Zettl1, E.Mix2, G.Rothe3, H.-P.Hartung1
1 Dept. of Neurology, Universty of Würzburg, 2 Dept. of Neurology, Hosp. Nerv. Dis., University of Rostock, 3 Dept. of Clinical Chemistry, University of Regensburg, Germany


Questions and comments to:
G.K.Valet, Max-Planck-Institut für Biochemie, Am Klopferspitz 18a, D-82152 Martinsried, Germany, Tel: +49/89/8578-2518, -2525, Fax: +49/89/8578-2563, E-mail: valet@vms.biochem.mpg.de
INTERNET address: http://www.biochem.mpg.de/valet/dgz.html
Last edit: Feb.18, 1996