The most recent version of this tutorial exists on the web and can be accessed directly if you are connected to the internet by pressing this symbol http://cmgm.stanford.edu/~roederer/compensation. If you are not on the internet press to run the tutorial now on the CD ROM!
There are two main "threads". The informal discussion that is aimed at the less technically oriented operators as well as a more rigorous treatment that provides additional examples and some math as well.
Tutorial on fluorescence compensations, multi-color flow cytometry. 49 individual slides in powerpoint format which can be used for teaching, lectures, and study material.
Discusses the intricacies of properly setting the compensation for
multiple-color analyses on flow cytometers.
Author: Geoffrey Osborne,
John Curtin School for Medical Research, Canberra, Australia.
osborne@anu.edu.au.
Set 2: Compensation Tutorial # 2
(2:MAC:COMP2SEA.HQX)
Discusses the intricacies of properly setting the compensation for
multiple-color analyses on flow cytometers.
Author: Geoffrey Osborne,
John Curtin School for Medical Research, Canberra, Australia.
osborne@anu.edu.au.
Set 3: Statistics Tutorial
(2:MAC:STATSSEA.HQX)
Discusses statistics used in flow cytometry.
Author: Geoffrey Osborne,
John Curtin School for Medical Research, Canberra, Australia.
osborne@anu.edu.au.
Set 4: Shutdown Protocol Tutorial
(2:MAC:SHUTPROT.HQX)
Discusses statistics used in flow cytometry.
Set 5: CellQuest Tutorial
(2:MAC:CQ_SEA.HQX)
A demonstration of the CellQuest for the Macintosh software.
Author: Geoffrey Osborne,
John Curtin School for Medical Research, Canberra, Australia.
osborne@anu.edu.au.
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