De Montfort University
Norman Barlaug Centre for Plant Science
Institute of Experimental Botany
Sokolovska 6, CZ-77200 Olomouc (Czech Republic)
phone 0042/68-5228521
fax 0042/68-5228523
Internet DOLEZEL@RISC.UPOL.CZ
Flow Karyotyping
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a chromosome suspension obtained by chopping of formaldeyde fixed root tips of field bean (Vicia faba L.).
Author: Jaroslav DOLEZEL, Sergio LUCRETTI
univariate flow karyotype
obtained after the analysis of a DAPI-stained chromosome suspension prepared from the EF line of V. faba (2n=12, reconstructed karyotype with stable translocations, Ingo Schubert, Institute of Plant Genetics and Crop Plant Research, Department of
Cytogenetics, D - 0 64 66 Gatersleben, Germany). All chromosome types can be discriminated and sorted.
Author: Sergio LUCRETTI, Jaroslav DOLEZEL.
univariate flow karyotype
obtained after the analysis of a DAPI-stained chromosome suspension prepared from V. faba with a standard karyotype. Peaks corresponding to chromatids of acrocentric chromosomes, five pairs of acrocentric chromosomes and a pair of metacentric chromosomes are well resolved.
Author: Sergio LUCRETTI, Jaroslav DOLEZEL
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This CD ROM was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette,
IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; EMAIL robinson@flowcyt.cyto.purdue.edu
a chromosome suspension obtained by chopping of formaldeyde fixed root tips of field bean (Vicia faba L.).
Author: Jaroslav DOLEZEL, Sergio LUCRETTI
univariate flow karyotype
obtained after the analysis of a DAPI-stained chromosome suspension prepared from the EF line of V. faba (2n=12, reconstructed karyotype with stable translocations, Ingo Schubert, Institute of Plant Genetics and Crop Plant Research, Department of
Cytogenetics, D - 0 64 66 Gatersleben, Germany). All chromosome types can be discriminated and sorted.
Author: Sergio LUCRETTI, Jaroslav DOLEZEL.
univariate flow karyotype
obtained after the analysis of a DAPI-stained chromosome suspension prepared from V. faba with a standard karyotype. Peaks corresponding to chromatids of acrocentric chromosomes, five pairs of acrocentric chromosomes and a pair of metacentric chromosomes are well resolved.
Author: Sergio LUCRETTI, Jaroslav DOLEZEL