The Purdue Cytometry CD ROM

List-Mode Files

Listmode files contributed by Hazel Davey.
Please be sure to acknowledge use of these data in any publication.
WARNING: You may receive an

error message when attempting to download any file from this CD ROM. The warning relates to the fact that the computer may try to READ the file. Clicking on "OK" will allow you to continue to download the file.

NOTE:The following files were collected on the Elite Cytometer with the following filter arrangement. Understanding this will aid interpretation of all of the .lmd files below:
pmt1 - 325nm excitation - all fluorescence + scatter <440nm detected
pmt2 - 488nm Side scatter
pmt3 - 525nm Fl from 488 ex
pmt4 - 575nm Fl from 488 ex
pmt5 - >600nm Fl from 488 ex
paramA - 525nm Fl from 325 ex

Choose a File to download

Filename yecfpt.lmd: Generated on the Coulter Epics Elite, this file shows Escherichia coli. The cells were fixed in 70% ethanol and stained with a cocktail of fluorescent brightener, fitc, and propidium iodide.
Filename yyfpt.lmd: Generated on the Coulter Epics Elite, this file shows Baker's yeast. The cells were fixed in 70% ethanol and stained with a cocktail of fluorescent brightener, fitc, and propidium iodide.
Filename 7ae2n.lmd: Generated on the Coulter Epics Elite, this file shows the bacterium Alkaligenes eutrophus which makes the biodegradable plastic poly-beta-hydroxy-butyrate. The cells were fixed in 70% ethanol and stained with nile red before analysis.
Filename 1bgyp.lmd: Generated on the Coulter Epics Elite, this file shows a mixture of Bacillus subtilis var. niger and Baker's yeast. The cells were fixed in 70% ethanol and stained with propidium iodide before analysis.

SKATRON FILES
Note: These files are also from Hazel Davey. They are readable by SkatGraf.exe which is listed in Eric Martz's free software catalogue.
Filename arg21003.dat: Generated on the Skatron Argus, this file shows a starved cultured of M. luteus stained with rhodamine.
Filename arg27012.dat: Generated on the Skatron Argus, this file shows a starved cultured of M. luteus after resuscitation, stained with rhodamine. (NB - The .DAT files are "SKATRON FORMAT" - not listmode.)

Go back to the LISTMODE FILE Index and select another set

CD HOMEPAGE | Table of Contents | Sponsor Home Pages | Sample Web Pages

This CD ROM was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; EMAIL robinson@flowcyt.cyto.purdue.edu

The Purdue Cytometry CD ROM

List-Mode Files

SKATRON FILES

Go back to the LISTMODE FILE Index and select another set