The Purdue Cytometry CD ROM
List-Mode Files
Listmode files contributed by Hazel Davey.
Please be sure to acknowledge use of these data in any publication.
WARNING: You may receive an
error message when attempting to download any file from this CD ROM. The warning relates to the fact that the computer may try to READ the file. Clicking on "OK" will allow you to continue to download the file.
NOTE:The following files were collected on the Elite Cytometer with the following filter arrangement. Understanding this will
aid interpretation of all of the .lmd files below:
pmt1 - 325nm excitation - all fluorescence + scatter <440nm detected
pmt2 - 488nm Side scatter
pmt3 - 525nm Fl from 488 ex
pmt4 - 575nm Fl from 488 ex
pmt5 - >600nm Fl from 488 ex
paramA - 525nm Fl from 325 ex
Choose a File to download
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Filename yecfpt.lmd: Generated on the Coulter Epics Elite, this file shows
Escherichia coli. The cells were fixed in 70% ethanol and stained
with a cocktail of fluorescent brightener, fitc, and propidium iodide.
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Filename
yyfpt.lmd: Generated on the Coulter Epics Elite, this file shows
Baker's yeast. The cells were fixed in 70% ethanol and stained
with a cocktail of fluorescent brightener, fitc, and propidium iodide.
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Filename 7ae2n.lmd: Generated on the Coulter Epics Elite, this file shows
the bacterium Alkaligenes eutrophus which makes the biodegradable
plastic poly-beta-hydroxy-butyrate. The cells were fixed in 70%
ethanol and stained with nile red before analysis.
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Filename 1bgyp.lmd: Generated on the Coulter Epics Elite, this file shows
a mixture of Bacillus subtilis var. niger and Baker's yeast.
The cells were fixed in 70% ethanol and stained with propidium
iodide before analysis.
SKATRON FILES
Note: These files are also from Hazel Davey. They are readable by SkatGraf.exe which is listed in Eric Martz's free software catalogue.
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Filename arg21003.dat: Generated on the Skatron Argus, this file shows
a starved cultured of M. luteus stained with rhodamine.
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Filename arg27012.dat: Generated on the Skatron Argus, this file shows
a starved cultured of M. luteus after resuscitation, stained with
rhodamine. (NB - The .DAT files are "SKATRON FORMAT" - not listmode.)
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This CD ROM was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette,
IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; EMAIL robinson@flowcyt.cyto.purdue.edu