IgM and IgG experiment
Tom Mchugh (mchugh@jekyll.ucsf.EDU)
Thu, 17 Feb 1994 17:42:14 -0800
There was e-mail regarding whether or not anti-IgG (heavy + light) would
detect a primary antibody which is IgM. I lost the header so I could not send
my reply directly. The second antibody of goat anti-mouse IgG (heavy + light)
will detect the first mouse IgM very well via the light chain. They should
either use heavy chain specific antibody or biotinylate one of them and
directly label the other. Even the heavy chain specific antisera, unless
heavily absorbed for light chains and other heavy chains may have some
reactivity to unexpected Ig's.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
