[Q] ELF, TOTO/TOPRO, AMCA

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I have several questions.
Could you reply some of them, if possible ?

1. Can ELF from Molecular Probes be used in flow cytometry ?
I want to stain intracellular proteins with AP-conjugated antibodies.
Amplification of the signals using ELF is available ?
Our EPICS ELITE ESP is equipped with He-Cd 325nm line. I think ELF
would be excited by 325nm UV.

Or, must I try to use Fast Red TR or something other substrates ?

2. Are TOTO-3 and TO-PRO-3 excited by Ar 488nm line ? I mean, when
analysing cell surface antigens by FITC, PE and PerCP-labeled
antibodies, can TOTO-3 and TO-PRO-3 be used as live/dead cell
discriminators like PI using He-Ne 633 nm simultaneously ?

3. Is there anybody who uses AMCA ?
Is this dye good for three or four color analysis ?

Any comments and replies would be appreciated.
Thank you.

--------------
Fumio Ike, PhD. E-mail:fike@rkna50.riken.go.jp
Animal and Cellular Systems Laboratory TEL:+81-48-462-1111(ext5613)
The Institute of Physycal and Chemical Research (RIKEN) FAX:+81-48-462-4681

• Next message: darzynk@nymc.edu: "anti-Fas antibodies -Reply"
• Previous message: Hector Nolla: "Re: Anti-TCR cross-reactivity?"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu