AUTOKLUS, ANALYSE for PC's available

Eric Martz (emartz@microbio.umass.edu)
Tue, 10 Jan 1995 14:07:21 -0500 (EST)

Tom Bakker Schut, the author of two PC programs for listmode flow cytometry
data analysis, has kindly provided copies with source code. With his
permission, I have made them available by anonymous ftp. Get them in
BINARY mode in /pub/flowcyt/autoklus at flowcyt.bio.umass.edu.

These programs and source code files were provided by Tom in November, 1993
(AUTOKLUS) and January, 1995 (ANALYSE).

AUTOKLUS is a cluster analysis program and is published in Cytometry
(Cytometry 14:649-659, 1993). This version of AUTOKLUS was improved
over the published version, and includes a text manual KLUSMAN.TXT.
AUTOKLUS now reads the sample Coulter files included as DATAZIP.EXE (which
were provided courtesy Geoff Osborne, ANU, Canberra, Australia), and a few
new options were added. Richard A. Cox is making further improvements to
AUTOKLUS not included in the version available on our ftp server; contact
him at racox@aol.com.

The program ANALYSE is a general purpose listmode analysis program. The
published reference is "ANALYSIS: Software for graphical analysis of flow
cytometric list mode data", T.C. Bakker Schut, R.M.P. Doornbos and B.G. de
Grooth. Computers and Biomedical Research 27:83-96 (1994).

The Pascal souce code is included for both programs. However, the
pascal files alone can not be compiled and linked into a working program
because they make use of units of a commercial software package, called
`Turbo 5.0 Professional'(TurboPower Software, Colorado Springs, CO,
USA).

For further information, contact Dr. Schut c/o Dr. Richard Doornbos
at dbs@tn.utwente.nl.

Editorial comment: AUTOKLUS is unique to my knowledge in providing
cluster analysis in a free program able to run on commonly available
computers (PC's with VGA). ANALYSE can be compared with Joe Trotter's
WinMDI (in /pub/pc at flosun.salk.edu), since both provide general purpose
analysis capabilities. ANALYSE has less-detailed help built-in than
does WinMDI, and WinMDI benefits from being a Windows program (ANALYSE is
a DOS program, e.g., can print only on Paintjets). Unfortunately, neither
program comes with a tutorial. In my experience, ANALYSE is noticeably
slower to load a listmode file than is WinMDI. However, I have not
evaluated ANALYSE thoroughly and it undoubtedly has unique and useful
features. I would appreciate hearing from others who evaluate these
programs.

/*- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Eric Martz, Professor of Immunology emartz@microbio.umass.edu
Dept Microbiology Voice: 413-545-2325 FAX: 413-545-1578
Morrill IVN 203, Box 35720, Univ Massachusetts, Amherst MA 01003-5720
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -*/


Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu