These programs and source code files were provided by Tom in November, 1993
(AUTOKLUS) and January, 1995 (ANALYSE).
AUTOKLUS is a cluster analysis program and is published in Cytometry
(Cytometry 14:649-659, 1993). This version of AUTOKLUS was improved
over the published version, and includes a text manual KLUSMAN.TXT.
AUTOKLUS now reads the sample Coulter files included as DATAZIP.EXE (which
were provided courtesy Geoff Osborne, ANU, Canberra, Australia), and a few
new options were added. Richard A. Cox is making further improvements to
AUTOKLUS not included in the version available on our ftp server; contact
him at racox@aol.com.
The program ANALYSE is a general purpose listmode analysis program. The
published reference is "ANALYSIS: Software for graphical analysis of flow
cytometric list mode data", T.C. Bakker Schut, R.M.P. Doornbos and B.G. de
Grooth. Computers and Biomedical Research 27:83-96 (1994).
The Pascal souce code is included for both programs. However, the
pascal files alone can not be compiled and linked into a working program
because they make use of units of a commercial software package, called
`Turbo 5.0 Professional'(TurboPower Software, Colorado Springs, CO,
USA).
For further information, contact Dr. Schut c/o Dr. Richard Doornbos
at dbs@tn.utwente.nl.
Editorial comment: AUTOKLUS is unique to my knowledge in providing
cluster analysis in a free program able to run on commonly available
computers (PC's with VGA). ANALYSE can be compared with Joe Trotter's
WinMDI (in /pub/pc at flosun.salk.edu), since both provide general purpose
analysis capabilities. ANALYSE has less-detailed help built-in than
does WinMDI, and WinMDI benefits from being a Windows program (ANALYSE is
a DOS program, e.g., can print only on Paintjets). Unfortunately, neither
program comes with a tutorial. In my experience, ANALYSE is noticeably
slower to load a listmode file than is WinMDI. However, I have not
evaluated ANALYSE thoroughly and it undoubtedly has unique and useful
features. I would appreciate hearing from others who evaluate these
programs.
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Eric Martz, Professor of Immunology emartz@microbio.umass.edu
Dept Microbiology Voice: 413-545-2325 FAX: 413-545-1578
Morrill IVN 203, Box 35720, Univ Massachusetts, Amherst MA 01003-5720
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