NK markers for baboons

David L. Haviland, Ph.D. (CLIVEG@niv.ac.za)
Sat, 18 Feb 1995 17:18:12 GMT+0200

In answer to Jim Henthorn

Jim

I have had extensive experience of measuring NK cells in the chacma
baboon. The human monoclonals we have used are: BD Leu 11b (anti-CD16)
which cross-reacts well; Coulter NKH1A (anti-CD56) or BD Leu 19 (anti-
CD56) which both cross-react nicely, the latter possibly more so. For
CD11b use Coulter Mo1 (it may be Mo2, I get confused). None of the
anti- CD57's I've tried work at all. For activated NK cells which
will express increased CD38, I've used OKT10 and it works well. I
have also found that the directly conjugated antibodies don't work as
nicely as the purified forms

I hope this helps

Clive Gray PhD
University of the Witwatersrand
Johannesburg
South Africa

e-mail: cliveg@niv.ac.za


Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu