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Aki:
Way back in Grad School, I was using an Epics V and was also trying to
examine cell cycling in T cells. I tried using the Hoechst (sp?) 33342 (also
maybe the 33258, but I really do think it was the 33342 - even after 9
years!). Needless to say, I was unable to get a classic growing cell cycle
histogram with Hoechst stains. THE only thing I did accomplish was being
able to roll the Epics in UV mode in about 20 minutes - that was all.
To complete the project, we wound up using Propidium Iodide via a fixation,
triton, RNAse like protocol. That gave us the picture purfect curve we
wanted to see...
Hope this helps,
David
>Aki Hoji (wondering when spring comes here)
>University of Wisconsin, Madison.
*wishing* for big snow but dissapointed at yet another wimpy mid-west
winter here in STL!
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