Re: AO DNA/RNA
brucem@cbme.unsw.edu.au
Wed, 17 May 95 09:39:55 +1000
Kirk,
There is a single-step method for AO determination of DNA/RNA in Taylor, I.W. and Milthorpe, B.K.
An evaluation of DNA fluorochromes, staining techniques and analysis for flow cytometry: 1. unperturbed cell populations.
J. Histochem. Cytochem. 28; 1224, 1980.
It works well on lymphocytes, but the conditions are fairly critical, as with
all AO methods.
Give it a go and email me if it doesn't work!
Bruce Milthorpe
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
