AO-EB filters

Dan Smith (DJS@ALLP.COM)
Thu, 29 Feb 96 16:40:00 PST

We are using the AO-EB combination staining of nuclear material to access
cells for apoptosis. We have a fluorescent scope but the filter sets we have
are specific for emissions of either 525nm OR 575nm (FITC or PE
respectively). What excitation/emission filter set should be used and would
you recommend to see both the green nuclear stain of AO in live cells and
the EB stain in the dead cells at the same time? A 525nm Band Pass?
Thanks /
Dan Smith
djs@allp.com

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