AFC assay.

• Messages sorted by: [ date ][ thread ][ subject ][ author ]
• Next message: Mario Roederer: "Compensation URL"
• Previous message: Mario Roederer: "Compensation & the WWW"

Does anyone have any experience in assaying intracellular
benzyloxycarbonyl tetra peptide AFC (7-Amino-4-Trifluoromethyl
Coumarin substrates.A few simple questions follow.

1 Are these compounds generally cell permeable

2 Can the cleaved AFC be excited at 352 nm , if so what is
the emission wavelength.

3 Does the cleaved AFC diffuse out of the cell.

Thanks for any help

Roger Snowden

rts1@le.ac.uk

• Next message: Mario Roederer: "Compensation URL"
• Previous message: Mario Roederer: "Compensation & the WWW"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu