Paraformaldehyde fixative
T. Scott Thurmond (thurmons@ehsct7.envmed.rochester.edu)
Sat, 27 Apr 1996 13:50:26 -0400
To the list,
Does anyone have a favorite recipe for paraformaldehyde as a
fixative for membrane antigens? I have gotten recipes from several
sources which range from the ridiculously complex to the ludicrously
simple (i.e., 1% paraformaldehyde in Dulbeccos's PBS). My question is
what should I take into account when selecting a fixative recipe and
what should I avoid (I will be fixing peritoneal cells for B
lymphocyte markers).
Any input would be appreciated.
Scott Thurmond
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
