How to get a monocellular cell suspension?

mariangela mancini (mmancini@fred.fhcrc.org)
Fri, 16 Jun 95 15:06:00 PDT

Hi! I am inducing differentiation in an anaplastic carcinoma cell line
using tyrosine kinase inhibitors in vitro. After treatment the cells
differentiate and form desmosomes. I harvest them for cell cycle analysis
and sorting, using trypsine (0.05%) with EDTA (up to 5 mM), but I still do
not obtain a monocellular cell suspension from my culture. After
trypsinization some of the cells are in suspension but some of them form
big clumps and the desmosomes are still there! Does anybody has any
suggestion about that?

Thanks!

Mariangela Mancini
e-mail mmancini@fred.fhcrc.org

Fred Hutchinson Cancer Research Center
Seatlle,WA

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