Re: Life expectancy of FACS nozzles

Dennis_Young@CIS.ucsd.edu
Wed, 19 Jul 1995 15:44:00 -0700

I think ceramics last for eons. You may need to sonicate (in the water bath
type) the nozzles to get the biological gunk off. If you have an inverted
'scope, you can see the effects of before and after sonication.

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* Dennis J. Young Voice : (619) 822-0407 *
* Flow Cytometry Core Facility FAX : (619) 822-0412 *
* University of California, San Diego USA e-mail: djyoung@ucsd.edu *
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*-------------------- FLOW CYTOMETRY FACILITY

Hello again,

Thank you to all who responded to my inquiry into protein secretion.

This is a question I have been wanting to ask, but always forget when I
send a posting.

Is there a life expectancy for the nozzles on the FACS Vantage (FACstar Plus)?
We have had our Vantage for about two years and have three 70um tip that I'm
unable to get tight CV's and short stream break off points for sorting. I'm
I not cleaning the nozzle good enough? or do the nozzles wear down where their
orfice is rough and produces turbulence.These nozzles are very expensive and
any response would be appreciated.

Thank you in advance.

Davin Jutila

DAVIN JUTILA, DIRECTOR SIU SCHOOL OF MEDICINE
FLOW CYTOMETRY FACILITY-1220 SPRINGFIELD, IL 62702
PHONE: (217)-524-5812 FAX: (217)-524-3227
EMAIL: SM0973%SPRINGB@SIUCVMB.SIU.EDU

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>Date: Wed, 19 Jul 95 11:47:21 CST
>From: DAVIN JUTILA <SM0973%SPRINGB.SIUCVMB@SIUCVMB.SIU.EDU>
>Subject: Life expectancy of FACS nozzles
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