DNA ANALYSIS OF SPERM
SUSAN DAWES (DAWES.SUSAN@EPAMAIL.EPA.GOV)
Thu, 03 Aug 1995 16:15:13 -0400
We are interested in staining fish sperm with PI to be used as an
additional ploidy marker in analyzing fish testis homogenates for DNA
content. Can anyone help with a successful method to preserve long-
term (freeze) aliquots of sperm to be prepared and run along with
other samples at a later date? Up to this point, all methods have
resulted in loss of cells following freezing in liquid nitrogen.
Also, any other hints for performing flow analysis of sperm would be
greatly appreciated. Thanks in advance! Sue
Susan Dawes
DynCorp/TAI
USEPA Cincinnati
DAWES.SUSAN@EPAMAIL.EPA.GOV
voice (513)569-7721
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
