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My Elite has a dichroic filter that excludes the UV line from the side
scatter detector. To get wavelength independent side scatter, I made an
angled reflector using a glass cover slip glued into a spare filter holder.
This directs a small proportion of the light into the side scatter detector
and lets the rest through to the other detectors. It works just fine and
also gives a side scatter signal with the red HeNe laser.
I assume that some arrangement like this is possible on most machines.
The Elite has a 488nm filter ahead of the forward scatter detector. This
must be removed when working at other wavelengths.
Good Luck,
Dean R. Hewish, Cell Biologist & Flow Cytometrist. ( and Amateur Malacologist)
CSIRO Biomolecular Engineering, 343 Royal Parade, Parkville, 3052,
Victoria Australia.
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