CellQuest problems
DrBlood (DrBlood@medschool.med.utah.edu)
Tue, 07 Nov 1995 15:49:54 +0800 (U)
CellQuest problems
MaryAnn DeMaria wrote:
>However, when we went
>to analyze the samples, 3 out of the 15 samples had
>randomly changed all the
>parameters. FL4 and FL5 staining was lost, FSC and
>SSC even were lost. We
>discovered that FL4 was now in FL2 and FL5 was in FL4
>and we could not find FSC
>or SSC. We can't understand this because all samples
>were run at the same time
>and it was not done in 3 consecutive samples but rather
>at random.
> This happened 2 days in a row.
I was remembering that we had a similar
problem and that we were advised by BD to
save data to the computer's hard drive at
the time of collection rather than to an
external optical disk to avoid this kind of
problem. We transfer data from the hard
drive to a server or to optical disks after
the data collection. We have not seen the
problem since starting this routine.
Jerry Spangrude
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
