fixing and storing cells
Ruedi Braun (rmbraun@ucdavis.edu)
Tue, 11 Jun 1996 21:03:26 -0700 (PDT)
Does anyone have experience with the fixation of cells after staining
with FITC or PE?
I am using 1% paraformaldehyde in PBS and analyze the cells within 12
hours after fixation.
We are planing to do some experiments with lavages on humans at high
altitude and I won't be able to get to a FACS for at least 5 days. Is
there a possiblity to fix stained cells for such a long time?
Many thanks for any help in this regard.
Ruedi
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