counterstain for bacteria

fAd4g!R0B&1ah: (100731.1123@CompuServe.COM)
21 Jun 96 03:50:26 EDT

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Hello Flowers!
we are new in this field. For FISH-applications we are looking for the perfect
combination of dyes.
Because our Flow-Cytometer (FACSCALIBUR, Argon-laser 488 nm) has no UV-laser, it
is not possible to stain the bacteria using DAPI (the usual counterstain).
On the other hand, we got perfect results using SYTO16 or SYTOX (Molecular
probes). But both dyes interfere with FITC (the usual probe-"stain").
Does anybody have an idea?
Kind regards

Gunnar Gerdts
Dept. Marine Microbiology
Biologische Anstalt Helgoland
Germany

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu