We are using a BD FACScan. We are currently using Gentrak lyse/fix, although I
think we're going to try the BD stuff.
I'm looking simply at what changes occur in the basic lineages (mono, gran,
lymph) when the mice are treated with novel proteins. I am using a three tube
panel: isotype control, B220/CD3 and CD14/Gr.1 (two color).
I'm more familiar with human LWB; the mouse FSC v. SSC looks similar but the
monocytes aren't as clearly separated--is this usually the case with mice?
Any mouse flow hints would be great,
Thanks,
Jeff Carrell
e-mail:
jeff_carrell@hgsi.com