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> I have an investigator who has been obtaining peritoneal macrophages from
> mice by lavage, culturing them overnight with TNF, and then looking by
> flow for Ia expression. We weren't getting much expression so I had him
> stain with PI at the same time. It turns out that the vast majority of
> the cells are dead. He has tried culturing in suspension or letting the
> cells adhere and using cold to detach them. Does anyone have a method of
> detaching that is easier on the cells and still leaves surface markers
> intact? Any advice is greatly appreciated.
>
> Carol
>
> -------------------------------------------------------------------------
> ---- Carol Oxford _| _| _|_|_|_|
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*Peter Openshaw (p.openshaw@ic.ac.uk) Direct:0171 594 3854 *
*Respiratory Medicine Switch:+44 171 723 1252 x 3854/3 *
*St. Mary's Hosp. Med. School Fax:+44 171 724 7349 *
*Imperial College, Norfolk Place, London W2 1PG, UK *
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