RE: Re: FW: BAL and clogging
vanburen%flovax.dnet.wayne.edu@rocdec.roc.wayne.edu
Tue, 9 Jul 1996 16:15:16 -0400
Simon Monard noted:
> 50u mesh sounds a bit big for a 70u nozzle.
Indeed, BDIS recommends using nozzles with a diameter that is four times
larger than the diameter of the largest particle sorted! A 200 micron tip
is available for the MacroSORT option (largest nozzle is 400 microns). For
a 70 micron tip, I use a 20 micron mesh; admittedly not quite 4:1, but at
least it gets out the visible clumps, pieces of tissue, etc. :)
/\/\/\_ Eric Van Buren, vanburen%flovax.dnet@rocdec.roc.wayne.edu
\ \ \ Karmanos Cancer Institute and Wayne State University,
\_^_/ Immunology & Microbiology. Detroit, Michigan.
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