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>Brian Rabinovitch enquired about staining for intracellular cytokines.
>
>Margaret North and John Farrant, Royal Free Hospital School of Medicine,
London,
>have supplied material for my CD-ROM ('Data Analysis in Flow Cytometry - a
>dynamic approach' available from Phoenix Flow Sytems) showing staining of
>mononuclear cells from human peripheral blood. The cells were incubated for
12 h
>either with monensin (unstimulated) or with phorbol myristate acetate (PMA),
>ionomycin and monensin (stimulated). They stained for IFN-gamma, TFN-alpha and
>IL-2. Their listed data files are on the CD-ROM. The accompanying book gives
>details of the method.
I do not mind people mentioning their own products, as long as they give
some useful information as well. In my opinion the above message does not
contain enough detailed information. Concentrations and sources of reagents,
and methodology are omitted. I urge the moderators of this cytometry list
not to include these COMMERCIAL MESSAGES!
Marcel Kuiper
King's College School of Medicine and Dentistry
Department of Molecular Medicine
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