Ca++ indicators
auger@ux1.cso.uiuc.edu
Tue, 8 Dec 92 14:25:13 CST
Greetings to all -
I have a quick question triggered by Steve Young's recent posts regarding
Indo-1 and Ca++ measurments. What is the general feeling for the "best"
fluorescent indicator in PBL's or cultured T-cell lines? Is Indo-1 the
current indicator of choice? Is it the best probe to use to look for T cell
activation/response to stimulators? I have read about many of the new
probes being developed, especially those excited in the visible spectrum,
and am curious if they would be better and easier to use than the Indo-1.
Any and all comments would be appreciated.
Thanks.
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University of Illinois at Urbana-Champaign auger@ux1.cso.uiuc.edu
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