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The MFI.EXE program includes extensive help including an introduction
to its capabilities.
Enhancements since 3.2 include:
- Shortcut keystrokes have been added to the main configuration menu
to toggle graphics on/off, and to route output to printer vs. screen.
- When graphics are disabled, it is now possible to back up to the
previous file, or to jump to the configuration menu.
- When the total events/file intentionally varies within a series of
list-mode data files, the warning about this can now be disabled (see
the text output configuration menu).
- Some bugs were fixed, and some polishing was done.
Users of version 3.2 may wish to keep a copy of this version (e.g.
MFI32.EXE) for use with previously analyzed groups of files. This
allows you to use your old gates, etc. If you run 3.3 in a directory
containing an old MFI.CFG, it will be renamed to MFIOLD.CFG. If you
wish to preserve the old configuration, simply copy MFIOLD.CFG back to
MFI.CFG and run MFI32.
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Instructions for retrieving MFI.EXE (and MSHERC.COM if you use Hercules
monochrome graphics) from Purdue. These instructions are for a unix
OS computer -- details may vary for other systems.
At your internet-connected computer, issue the command
"ftp flowcyt.cyto.purdue.edu".
If the FTP command is functional on your computer, you will be asked
to logon. Give the name "anonymous". For a password, give your
complete email address. You will "arrive" at Purdue in the directory
pub. The command "pwd" (print working directory) will confirm this.
Issue the command "cd martz" to change to pub/martz. The command "ls"
produces a directory listing from which you can decide which files to
"get". Typically, issue the command "get mfi.exe" (and if you need
it, "get msherc.com"). You can see what else is available by getting
martz.doc, which also contains the dates of all upgrade postings.
Should you wish to upload any list mode data files so that I can try
to make MFI compatible with them, use the commands "cd .." (returning
you to /pub), "cd upload" (putting you at pub/upload), then "send
filename". (The file to be sent should be available in the local
directory from which you called ftp in the first place.) You may ARC
or ZIP files first if you wish.
When finished, say "bye" to ftp.
If you do not have access to an internet-linked computer which
supports FTP, ask your friends. This method is best because MFI is
improved frequently and by FTP you can always have the latest version
quickly. From our local unix computer, it takes less than 4 seconds
to get or put MFI.EXE between Mass. and Indiana! If FTP is not
accessible to you, I may be able to email you the program in uuencoded
form (be sure you have uudecode available before you ask me to do
this). This is more time consuming at my end, especially for overseas
mailings which may have to be split into 5 or so pieces to satisfy the
50K limit, then re-assembled by hand editing at your end. Within the
USA, it usually seems possible to email the uuencoded MFI.EXE in one
piece (about 210K). Snail mail of floppy diskettes is time consuming
at my end and by the time the program arrives it is usually obsolete.
--
/* - - - - - - - - - - - - - - - - - From - - - - - - - - - - - - - - - - - -
Eric Martz emartz@titan.ucc.umass.edu Professor of Immunology
Voice 413-545-2325, FAX 413-545-1578
Morrill IVN Rm 203, Dept Microbiol, Univ Mass, Amherst MA 01003 USA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - */
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