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One of my users has been looking at DNA content in fish RBC's for a long
time (they are nucleated). She recently started to work on the leukocyte
side of things, and has gotten great results (CV's < 2.0) with spinning
through F-H, staining the cells w/ PI (modified Vindolov) and then
freezing the cells for later flow analysis (this staining will be done in
the field [Russia] if the method seems reliable). Her major professor is
amazed that this works, and knowing of my network connection, he wanted me
to ask if anyone else out there has tried this sort of thing? I realize
this is out on the fringes, but can anyone think of any criticisms or
warnings other than my flippant response ("hey, if it works, why
complain?"). Can anybody see any potential problems with this approach?
Thanks for any feedback,
steve
Steve Hilliard : "Be good, and you will be
Cell Analysis Facility, Univ. of GA : lonesome."
email: hilliard@zookeeper.zoo.uga.edu :
voice: (706)542-9474 : I brew the beer I drink!
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