Re: 4 color flow
Kai Toellner (TOELLNKM@novell1.bham.ac.uk)
Fri, 1 Dec 1995 10:04:13 BST
> Begin forwarded message:
>
> Date: Wed, 29 Nov 1995 08:52:33 +0800
> X-Sender: nmilkh@leonis.nus.sg
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> From: nmilkh@leonis.nus.sg (KH Leong)
> Subject: Re: 4 color flow
> Cc: "David L. Haviland, Ph.D." <HAVILAND@kids.wustl.edu>
>
> ...Although we can detect PerCP on the EPICS, we have not been able to do so
> on our FACStarPLUS! We are still waiting to hear from BD...and meanwhile we
> are trying to figure out the sets of filters and dichroic mirrors for the
> FACStar+. Maybe you or someone else out there could help?
>
> ------
>
> PerCP is very photolabile. 50mW of 488nm light is enough to kill the
> fluorochrome in my experience. Those who have reported that it works have
> used either air-cooled argon lasers or N.D. filters to decrease the power
> output of a water-cooled laser. A 670 band pass filter will work just fine
> for detection of PerCP fluorescence.
>
> Dave Coder
> dcoder@u.washington.edu
>
Dyes which work very well with the FACStarPlus are RED613 and RED670
from Gibco.
Kai Toellner
----------------------------------------------------------------------
Dr. Kai-Michael Toellner
Department of Immunology
The Medical School Tel.: (44) 121 414 6970
University of Birmingham FAX.: (44) 121 414 3599
Birmingham B15 2TT, UK E-mail: K.M.Toellner@bham.ac.uk
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