PKH linker dyes

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Does anyone know if the use of these dyes, PKH26 and PKH2, in stimulated
human peripheral blood cell cultures will interfer with the expression of
activation markers? Is there leakage and cell toxicity problems with cultures
up to 5 days?

Thanks in advance.

Vivian Wu
Immunology Lab
Vancouver Hospital & HSC
B.C.
Email: ffwu@unixg.ubc.ca

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu