Phenotype for CML cells
Roger Burger (Roger@cpd2.usu.edu)
Mon, 11 Dec 1995 12:21:03 -0700
I have recently been asked to work with some peripheral blood cells from a
bone marrow transplant recipient with CML. I would like to be able to
isolate the leukemic cells from the other leukocytes and was wondering if
someone could tell me if these cells have a specific phenotype I can look
for. I suspect that these cells are CD34+ DR+ and at some point following
blast crisis they express CD19 but I am not to sure on this and hope that
one of you working in a clinical stetting can help me with this.
I also want to culture these cells in vitro and have a 1993 paper by Morel,
et al, suggesting the use of sCD23 and IL-7 in culture. I plan to use the
CML cells as stimulators for CTL and eventually targets for measuring CTL
reponse. I will need to keep them in culture for several weeks. Again any
ideas on this topic would be greatly appreciated.
Thanks
***********************************************
Arly A. Helm, nutritionist, lactation consultant email: arly@cc.usu.edu
Roger A. Burger E-mail: Roger@cpd2.usu.edu
Research Immunologist
Center for Persons with Disabilities
Utah State University
Logan, UT 84322-6800
Voice: 801-797-2042
FAX: 801-797-2044
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
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EMAIL robinson@flowcyt.cyto.purdue.edu
