F-actin
DR M AL-RUBEAI (ALRUBEAM@novell1.bham.ac.uk)
Wed, 13 Dec 1995 11:34:17 BST
I have been treating hybridoma cells grown in suspension with
cytochalasin D to disrupt the F-actin cytoskeleton prior to staining
with FITC-phalloidin. Cells treated with cytochalasin seem to have a
higher mean fluorescence than untreated cells, this I believe is due
to cytochalasin induced clumping of the F-actin into dense aggregates
that fluoresce more brightly than the difuse F-actin network of
untreated cells. Has anyone noticed a similar result? If so, is it
possible to use this as a quantitative measure of disruption of the
F-actin by cytochalasin?
Thank you
Jon Welsh
University of Birmingham
School of Chemical Engineering
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