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On Thu, 7 Dec 1995, Susan & Ulrik Sprogxe-Jakobsen wrote:
> Hello out there!
>
> I am performing flow cytometry analysis on human red cells. Due to the low
> number of antigen copies per cell, the resulting fluorescense (FITC) is
> close to the flowcytometer (FACScan) noise level.
>
> 1. Does anyone know how to calculate the real number of cell-bound FITC
> molecules (and hence antigens), when working in this low area of
> fluorescence intensity?
>
> 2. Unmarked human red cells have a lower green (FITC) autofluorescence
> than certified blank beads. Any suggestions on how to incorporate this in
> the calculations of cell-bound FITC molecules on human RBCs?
>
>
> Any suggestion appreciated ...
>
> Best regards
>
> Ulrik Sprogoe-Jakobsen
>
> Dept. Clinical Immunology
> Odense University Hospital
> DK-5000 Odense C
> Phone: +45 6541 3576
> Fax: +45 6612 7975
>
>
>
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