Re[2]: FACScan side scatter problems

/G=Gerhard/S=Nebe-von-Caron/OU=1890CHPE/O=TMGB.URC/@LANGATE.gb.sprint.com
Wed, 20 Dec 1995 03:01:00 -0500

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The problem described sounds a bit like air bubbles. Using a
logical scatterplot (y-axis=forward scatter or size and
x-axis=90'scatter or granularity) air bubbles lean to the
y-axis and show a spread of forward scatter but less side
scatter than lymphocytes (big but hollow) whilst
precipitates or crystals lean to the x-axis (small but
glitter like). A possible cause might be air that gets in
through the sample insertion rod. See if the problem appears
more frequent on lower sample flow or with low sample
volumes which increase the likelihood of air getting trapped
not being flushed through ahead of the sample. Check the
o-ring seal.

Gerhard Nebe-v.Caron
Unilever Research, Colworth Laboratory Sharnbrook,
Bedfordshire
GB - MK44 1LQ
Tel: +44(0)1234-222066
FAX: +44(0)1234-222344
E.mail: gerhard.nebe-von-caron@urcgb.sprint.com

______________________________ Reply Separator _________________________________
Subject: Re: FACScan side scatter problems
Author: Karenrt@aol.com at INTERNET
Date: 19/12/95 13:56

I've seen this problem too. It appears to be "electronic noise" coming
through your institution's electrical supply lines. We also consulted BDIS
and they could not help. They recommended a more expensive surge
suppressor/power strip, which we bought, but it did not help. Perhaps a
really good line tamer would help? Otherwise, setting a gate to ignore this
stuff or restarting acquisition seem to be the only current solutions. Hope
someone else has a better idea!

Karen Tamul
FAST Systems, inc

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu