re: Indo loading

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I haven't actually used COS cells, but we had problems loading CHO cells,
which pump indo-1 out. You should definitely try using the Pluronic, though
it might not help much. Verapamil will probably help a lot, but it is a
blocker of voltage-gated calcium channels. That probably won't matter for
your cells, but you should be aware of that. We usually load CHO cells while
they are attached, but they can also be loaded in suspension. We ended up
isolating a mutant CHO cell line that loads well with indo to do our studies
(see report in Cytometry 17:33-38, 1994). Hopefully you don't have to take
such an extreme measure.

Mike Fox
Colorado State University

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• Maybe in reply to: PLOPEZ@sorter.dfci.harvard.edu: "Indo loading"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu